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IL1A 抗体 (Biotin)

The Biotin-conjugated 兔 多克隆 anti-IL1A antibody (ABIN465868) specifically detects IL1A in WB, ELISA 和 Neut. The antibody is reactive with 小鼠 samples.
产品编号 ABIN465868
发货至: 中国
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Quick Overview for IL1A 抗体 (Biotin) (ABIN465868)

抗原

See all IL1A 抗体
IL1A (Interleukin 1 alpha (IL1A))

适用

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小鼠

宿主

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克隆类型

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多克隆

标记

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This IL1A antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 特异性

    Mouse IL-1alpha

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Murine IL-1alpha.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

    Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mIL-1alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1alpha. To detect mIL-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-1alpha (LS-C104666) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1alpha. Western Blot: To detect mIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile PBS containing 0.1 % BSA

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    IL1A (Interleukin 1 alpha (IL1A))

    别名

    IL1A / IL1 Alpha

    背景

    Name/Gene ID: IL1A
    Family: IL-1

    Synonyms: IL1A, Hematopoietin-1, IL-1 alpha, IL1, IL1-ALPHA, IL1 Alpha, Interleukin-1 alpha, IL-1A, IL1F1, Pro-interleukin-1-alpha, Preinterleukin 1 alpha, Interleukin 1, alpha

    基因ID

    3552

    UniProt

    P01583

    途径

    NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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