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CNTF 抗体

CNTF 适用: 大鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN465750
发货至: 中国
  • 抗原 See all CNTF 抗体
    CNTF (Ciliary Neurotrophic Factor (CNTF))
    适用
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    大鼠
    宿主
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    克隆类型
    • 78
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    多克隆
    标记
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    This CNTF antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Neutralization (Neut)
    特异性
    Rat CNTF
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Rat CNTF.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product CNTF Primary Antibody
  • 应用备注
    Approved: ELISA (0.5 - 2 μg/mL), Neut (2 - 5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (including the globus pallidus region) tissue. The primary antibody was incubated at 0.25 mg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat CNTF (0.15 ng/mL), a concentration of 0.02-0.05 μg/mL of this antibody is required. ELISA: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat CNTF (LS-C104736) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western Blot: To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Rat

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    CNTF (Ciliary Neurotrophic Factor (CNTF))
    别名
    CNTF (CNTF 产品)
    别名
    GPA antibody, CNTF antibody, HCNTF antibody, AI429687 antibody, ciliary neurotrophic factor antibody, CNTF antibody, Cntf antibody
    背景
    Name/Gene ID: CNTF

    Synonyms: CNTF, Ciliary neurotrophic factor, HCNTF
    基因ID
    1270
    UniProt
    P26441
    途径
    JAK/STAT Signaling
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