IL1A 抗体 (Biotin)
Quick Overview for IL1A 抗体 (Biotin) (ABIN465287)
抗原
See all IL1A 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human IL-1alpha
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Human IL-1alpha.
Type of Immunogen: Cell extract
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应用备注
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Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hIL-1alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-1alpha. To detect hIL-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-1alpha (LS-C104630) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-1alpha. Western Blot: To detect hIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS containing 0.1 % BSA
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- IL1A (Interleukin 1 alpha (IL1A))
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别名
- IL1A / IL1 Alpha
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背景
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Name/Gene ID: IL1A
Family: IL-1
Synonyms: IL1A, Hematopoietin-1, IL-1 alpha, IL1, IL1-ALPHA, IL1 Alpha, Interleukin-1 alpha, IL-1A, IL1F1, Pro-interleukin-1-alpha, Preinterleukin 1 alpha, Interleukin 1, alpha -
基因ID
- 3552
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UniProt
- P01583
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途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
抗原
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