IGF1 抗体 (Biotin)
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- 抗原 See all IGF1 抗体
- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IGF1 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- Human IGF-I
- 纯化方法
- Immunoaffinity purified
- 免疫原
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E.coli derived recombinant Human IGF-I.
Type of Immunogen: Cell extract - Top Product
- Discover our top product IGF1 Primary Antibody
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- 应用备注
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Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: ELISA: To detect hIGF-I by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-I. To detect hIGF-I by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IGF-I (LS-B7414) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-I. Western Blot: To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Sterile PBS containing 0.1 % BSA
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- 抗原
- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
- 别名
- IGF-I / IGF1 (IGF1 产品)
- 别名
- IGF-I antibody, IGF1A antibody, IGFI antibody, C730016P09Rik antibody, Igf-1 antibody, Igf-I antibody, Npt2B antibody, IGF-1 antibody, IGFIA antibody, IGF-IB antibody, igf1 antibody, IGF-1L antibody, IGF-1a antibody, IGF1 antibody, igf-1 antibody, igf1-A antibody, igf1.S antibody, xigf1 antibody, insulin like growth factor 1 antibody, insulin-like growth factor 1 antibody, insulin-like growth factor I antibody, insulin like growth factor 1 L homeolog antibody, IGF1 antibody, Igf1 antibody, LOC100136741 antibody, igf1 antibody, LOC678666 antibody, igf1.L antibody, LOC104911603 antibody
- 背景
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Name/Gene ID: IGF1
Family: Hormone
Synonyms: IGF1, IGF-IA, IGF-IB, IGFI, IBP1, IGF-I, Insulin-like growth factor 1, Insulin-like growth factor I, Insulin-like growth factor IA, Insulin-like growth factor IB, Mechano growth factor, Somatomedin-C, IGF1A, MGF - 基因ID
- 3479
- UniProt
- P05019
- 途径
- RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
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