Collagen Type I 抗体
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- 抗原 See all Collagen Type I (COL1) 抗体
- Collagen Type I (COL1) (Collagen, Type I (COL1))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Collagen Type I antibody is un-conjugated
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应用范围
- ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- 品牌
- IHC-plus™
- 特异性
- Human collagen type. Cross-reactivity: Human collagen type I: 100 % , Human collagen type II: <0.5 %, Human collagen type III: < 25 %, Human collagen type IV: <0.5 %, Human collagen type V: <0.5 %, Human fibronectin: <0.5 %, Human albumin: <0.5 %, Human immunoglobulin: <0.5 %.
- 纯化方法
- Ammonium sulfate precipitation
- 免疫原
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Human placental collagen Type I.
Type of Immunogen: Purified protein - 亚型
- IgG
- Top Product
- Discover our top product COL1 Primary Antibody
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- 应用备注
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Approved: ELISA, IF, IHC, IHC-P (1:100)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3653 was determined to be 1:100. ELISA on human collagen type I: 1:4000. Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue: 1:10-1:40.
Not recommended for: WB - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- Lot specific
- 缓冲液
- Lyophilized, PBS, pH 7.5, 0.1 % Mannitol, 0.1 % Dextran.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- 抗原
- Collagen Type I (COL1) (Collagen, Type I (COL1))
- 别名
- Collagen I (COL1 产品)
- 别名
- collagen type III alpha 1 chain antibody, COL3A1 antibody
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