山羊 anti-兔 IgA, IgG, IgM (Fab Region), (Light Chain) Antibody - Preadsorbed
ELISA, WB, ICC
Polyclonal
IgG
unconjugated
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- 抗原 See all IgA, IgG, IgM products
- IgA, IgG, IgM
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抗原表位
- Fab Region, Light Chain
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
- 特异性
- The reactivity of the antiserum is directed to the major isotypes of the rabbit immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rabbit plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained.
- 产品特性
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Purified IgG fraction of polyclonal goat antiserum to rabbit immunoglobulins IgG, IgA, IgM, heavy and light chains
- 纯化方法
- Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
- 免疫原
- Highly purified IgG and pools of homogenous IgA and IgM isolated from rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
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The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted.
The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5,000. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
- 浓度
- 10 mg/mL
- 缓冲液
- Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 注意事项
- Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storag e at or below -24 °C.
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- 抗原
- IgA, IgG, IgM
- 别名
- IgG + IgA + IgM (IgA, IgG, IgM 产品)
- 物质类
- Antibody
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