Secretory Component 抗体 (Membrane-Bound, Soluble) (HRP)
适用: 人
ELISA, WB, IF
宿主: 小鼠
Monoclonal
NI 194-4 (A89-039)
HRP
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- 抗原 See all Secretory Component products
- Secretory Component
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抗原表位
- Membrane-Bound, Soluble
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Secretory Component antibody is conjugated to HRP
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应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
- 产品特性
- Horseradish peroxidase conjugated purified monoclonal mouse antibody to human secretory component, free and bound
- 纯化方法
- Purified
- 免疫原
- Highly purified secretory component isolated from human milk.
- 克隆位点
- NI 194-4 (A89-039)
- 亚型
- IgG1 kappa
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- 应用备注
- To identify the presence of lactoferrin in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immuno-peroxidase and indirect immunofluorescence staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards, in ELISA from 1:50 upwards, in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
- 缓冲液
- Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized product by addin g 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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- 抗原
- Secretory Component
- Abstract
- Secretory Component 产品
- 别名
- RNPIGR2 antibody, pIgA-R antibody, polymeric immunoglobulin receptor antibody, PIGR antibody, Pigr antibody
- 背景
- The reactivity of the antiserum is restricted to free and bound secretory component as tested in indirect binding immunoblotting and indirect immunoperoxidase staining.
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