CD3 抗体
(7 references)-
- 抗原 See all CD3 抗体
- CD3
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD3 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunocytochemistry (ICC)
- 特异性
- The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
- 交叉反应 （详细）
- Human, Mouse
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Purified human CD3 proteins isolated from thymus
- 克隆位点
- APA1-1
- 亚型
- IgG1
- Top Product
- Discover our top product CD3 Primary Antibody
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anti-CD3 (CD3) antibody
CD3 适用: 人, 小鼠, 大鼠 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, CyTOF 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, CyTOF 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, Func, CyTOF 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-57 Biotin
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (APC)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 APC
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP
anti-CD3 (CD3) antibody (PE)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PE
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- 应用备注
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Flow cytometry: Intracellular staining, recommended dilution: 1-4 μg/mL, positive control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/mL). Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1 % BSA for 20 min. on ice. Wash with PBS and fix with 2 % formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1 % saponine, 5 min. RT. Incubate the cells in PBS + 1 % BSA + 0.03 % saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 μg/mL) in PBS + 1 % BSA + 0.03 % saponine in dark, 20 min. RT. Wash with PBS + 1 % BSA + 0.03 % saponine, resuspend in PBS.
Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "The proline-rich sequence of CD3epsilon as an amplifier of low-avidity TCR signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 1, pp. 243-55, (2008) (PubMed).
: "Targeted cell replacement with bone marrow cells for airway epithelial regeneration." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 293, Issue 3, pp. L740-52, (2007) (PubMed).
: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, (2006) (PubMed).
: "T cell receptor engagement by peptide-MHC ligands induces a conformational change in the CD3 complex of thymocytes." in: The Journal of experimental medicine, Vol. 201, Issue 4, pp. 517-22, (2005) (PubMed).
: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, (2003) (PubMed).
: "CD3delta couples T-cell receptor signalling to ERK activation and thymocyte positive selection." in: Nature, Vol. 406, Issue 6794, pp. 426-30, (2000) (PubMed).
: "The CD3-gamma and CD3-delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes." in: The EMBO journal, Vol. 10, Issue 4, pp. 903-12, (1991) (PubMed).
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: "The proline-rich sequence of CD3epsilon as an amplifier of low-avidity TCR signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 1, pp. 243-55, (2008) (PubMed).
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- 抗原
- CD3
- 别名
- CD3 (CD3 产品)
- 别名
- CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody
- 背景
- CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 epsilon chain, T3E, IMD18
- 基因ID
- 916
- UniProt
- P07766
- 途径
- TCR Signaling, Ubiquitin Proteasome Pathway
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