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CD19 抗体

Name: CD19 抗体
Brand: antibodies-online
SKU: ABIN457399
Availability: OutOfStock

CD19 适用: 小鼠 FACS, IP, IHC (fro), Func 宿主: 大鼠 Monoclonal 1D3 unconjugated

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD19 (1D3) purified antibody (low endotoxin, concentration in sample 0,6 μg/mL) DAR APC. (CD19 抗体)

FACS

Separation of murine CD19 positive splenocytes (red-filled) from CD19 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD19 (1D3) purified antibody (low endotoxin, concentration in sample 0,6 μg/mL) DAR APC. (CD19 抗体)

FACS

2 images

(5 references)

产品编号 ABIN457399

发货至: 中国

Datasheet as PDF

抗原 See all CD19 抗体

CD19 (CD19 Molecule (CD19))
适用
293

161

48

30

19

10

7

6

3

3

2

2

2

2

2

2

2

2
小鼠
宿主
172

140

46

4
大鼠
Compatible Secondaries
克隆类型
230

128

2
单克隆
标记
111

22

21

19

16

8

8

8

7

7

7

7

7

7

7

6

6

6

4

4

4

4

4

4

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This CD19 antibody is un-conjugated
应用范围
262

116

72

62

59

39

23

19

17

14

14

8

5

4

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

特异性

The rat monoclonal antibody 1D3 detects an extracellular epitope of murine CD19, 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells, it is lost on plasma cells.

交叉反应（详细）

Mouse

纯化方法

Purified by protein-G affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

内毒素水平

Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

免疫原

Mouse CD19-transfected cell line

克隆位点

1D3

亚型

IgG2a

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anti-CD19 Molecule (CD19) (C-Term) antibody
CD19 适用: 人, 小鼠, 猴, 大鼠, 绵羊, Pig, Cow, 豚鼠, 犬, 驴, 山羊, Hamster, 马, 兔, Avian, Cat, 小鸡 WB 宿主: 山羊 Polyclonal unconjugated

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anti-CD19 Molecule (CD19) (AA 96-281) antibody
CD19 适用: 人 FACS, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CD19-3116 unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 适用: 人 FACS, WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 143-172) antibody
CD19 适用: 人 FACS, WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 160-258) antibody
CD19 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 20-291) antibody
CD19 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4C6G1 unconjugated

应用备注

Functional application: This antibody can induce down-regulation of CD19, affecting the proportions of B cell subpopulations.
Flow cytometry: Recommended dilution: 1-4 μg/mL

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4

储存液

Azide free

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Shoham, Rajapaksa, Boucheix, Rubinstein, Poe, Tedder, Levy: "The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 8, pp. 4062-72, (2003) (PubMed).

Inabe, Kurosaki: "Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement." in: Blood, Vol. 99, Issue 2, pp. 584-9, (2002) (PubMed).

Cherukuri, Cheng, Pierce: "The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 1, pp. 163-72, (2001) (PubMed).

Krop, de Fougerolles, Hardy, Allison, Schlissel, Fearon: "Self-renewal of B-1 lymphocytes is dependent on CD19." in: European journal of immunology, Vol. 26, Issue 1, pp. 238-42, (1996) (PubMed).

Krop, Shaffer, Fearon, Schlissel: "The signaling activity of murine CD19 is regulated during cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 1, pp. 48-56, (1996) (PubMed).
抗原

CD19 (CD19 Molecule (CD19))

别名

CD19 (CD19 产品)

别名

B4 antibody, CVID3 antibody, AW495831 antibody, CD19 antibody, CD19 molecule antibody, CD19 antigen antibody, CD19 antibody, Cd19 antibody

背景

CD19 Molecule,CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.,B4, Leu-12, Cvid

基因ID

12478

UniProt

P25918

途径

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway