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Apolipoprotein M 抗体 (C-Term)

APOM 适用: 人, 犬 IHC (p), EIA 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN452682
发货至: 中国
  • 抗原 See all Apolipoprotein M (APOM) 抗体
    Apolipoprotein M (APOM)
    抗原表位
    • 15
    • 8
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    C-Term
    适用
    • 49
    • 28
    • 15
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    人, 犬
    宿主
    • 59
    • 16
    • 2
    山羊
    克隆类型
    • 60
    • 19
    多克隆
    标记
    • 41
    • 15
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This Apolipoprotein M antibody is un-conjugated
    应用范围
    • 65
    • 34
    • 24
    • 13
    • 13
    • 10
    • 10
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    特异性
    This antibody recognizes Apolipoprotein M (Apo M).
    交叉反应 (详细)
    Species reactivity (expected):Canine.
    Species reactivity (tested):Human.
    纯化方法
    Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
    免疫原
    Peptide with sequence PRNQEACELSNN, from the C Terminus of the protein sequence according to NP_061974.2.
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  • 应用备注
    Peptide ELISA: Antibody Detection Limit Dilution: 1/8000. Western Blot: Preliminary experiments gave an approx 50 kDa band in Human Kidney andLiver lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 21.0 kDaaccording to NP_061974.2. We do note that ApoM is a glycoprotein which makes a highersize band to be expected. The 50 kDa band was successfully blocked by incubation withthe immunizing peptide. Immunohistochemistry: 3-6 μg/mL. In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    0.5 mg/mL
    缓冲液
    Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原
    Apolipoprotein M (APOM)
    别名
    Apolipoprotein M (Apo M) (APOM 产品)
    别名
    G3a antibody, HSPC336 antibody, NG20 antibody, apo-M antibody, 1190010O19Rik antibody, APOM antibody, fb62d12 antibody, wu:fb62d12 antibody, g3a antibody, ng20 antibody, hspc336 antibody, mKIAA4033 antibody, apolipoprotein M antibody, apolipoprotein M S homeolog antibody, myeloperoxidase antibody, APOM antibody, Apom antibody, apom.S antibody, apom antibody, Mpo antibody
    背景
    Apolipoprotein M is a member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. It is positively related to leptin. It is mainly expressed in liver and kidney. Apolipoprotein M is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. It is important for the formation of prebeta-HDL and cholesterol efflux to HDL, and thereby inhibits formation of atherosclerotic lesions.Synonyms: Apo-M, ApoM, HSPC336, NG20, Protein G3A
    基因ID
    55937
    NCBI登录号
    NP_061974
    UniProt
    O95445
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