Apolipoprotein M 抗体 (C-Term)
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- 抗原 See all Apolipoprotein M (APOM) 抗体
- Apolipoprotein M (APOM)
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抗原表位
- C-Term
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适用
- 人, 犬
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Apolipoprotein M antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody recognizes Apolipoprotein M (Apo M).
- 交叉反应 (详细)
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Species reactivity (expected):Canine.
Species reactivity (tested):Human. - 纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- 免疫原
- Peptide with sequence PRNQEACELSNN, from the C Terminus of the protein sequence according to NP_061974.2.
- Top Product
- Discover our top product APOM Primary Antibody
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- 应用备注
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Peptide ELISA: Antibody Detection Limit Dilution: 1/8000. Western Blot: Preliminary experiments gave an approx 50 kDa band in Human Kidney andLiver lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 21.0 kDaaccording to NP_061974.2. We do note that ApoM is a glycoprotein which makes a highersize band to be expected. The 50 kDa band was successfully blocked by incubation withthe immunizing peptide. Immunohistochemistry: 3-6 μg/mL. In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- Apolipoprotein M (APOM)
- 别名
- Apolipoprotein M (Apo M) (APOM 产品)
- 别名
- G3a antibody, HSPC336 antibody, NG20 antibody, apo-M antibody, 1190010O19Rik antibody, APOM antibody, fb62d12 antibody, wu:fb62d12 antibody, g3a antibody, ng20 antibody, hspc336 antibody, mKIAA4033 antibody, apolipoprotein M antibody, apolipoprotein M S homeolog antibody, myeloperoxidase antibody, APOM antibody, Apom antibody, apom.S antibody, apom antibody, Mpo antibody
- 背景
- Apolipoprotein M is a member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. It is positively related to leptin. It is mainly expressed in liver and kidney. Apolipoprotein M is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. It is important for the formation of prebeta-HDL and cholesterol efflux to HDL, and thereby inhibits formation of atherosclerotic lesions.Synonyms: Apo-M, ApoM, HSPC336, NG20, Protein G3A
- 基因ID
- 55937
- NCBI登录号
- NP_061974
- UniProt
- O95445
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