RHOT1 抗体 (N-Term)
(1 validation)-
- 抗原 See all RHOT1 抗体
- RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))
-
抗原表位
- N-Term
-
适用
- 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马, 斑马鱼
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This RHOT1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB)
- 序列
- EMKPACIKAL TRIFKISDQD NDGTLNDAEL NFFQRICFNT PLAPQALEDV
- 预测反应
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%
- 产品特性
- This is a rabbit polyclonal antibody against RHOT1. It was validated on Western Blot using a cell lysate as a positive control.
- 纯化方法
- Affinity Purified
- 免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human RHOT1
- Top Product
- Discover our top product RHOT1 Primary Antibody
-
anti-Ras Homolog Gene Family, Member T1 (RHOT1) (N-Term) antibody
RHOT1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Ras Homolog Gene Family, Member T1 (RHOT1) (N-Term) antibodyRHOT1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ras Homolog Gene Family, Member T1 (RHOT1) (AA 483-580) antibodyRHOT1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4H4 unconjugated
anti-Ras Homolog Gene Family, Member T1 (RHOT1) (N-Term) antibodyRHOT1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Ras Homolog Gene Family, Member T1 (RHOT1) (N-Term) antibodyRHOT1 适用: 人, 小鼠, 大鼠, 兔, Cow, 豚鼠, 马, Bat, 小鸡, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ras Homolog Gene Family, Member T1 (RHOT1) antibodyRHOT1 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal CL1083 unconjugated
anti-Ras Homolog Gene Family, Member T1 (RHOT1) (AA 483-580) antibodyRHOT1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1A12 unconjugated
anti-Ras Homolog Gene Family, Member T1 (RHOT1) (AA 483-580) antibodyRHOT1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ras Homolog Gene Family, Member T1 (RHOT1) (AA 396-445) antibodyRHOT1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马, Bat, 小鸡, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Ras Homolog Gene Family, Member T1 (RHOT1) (AA 1-659) antibodyRHOT1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
-
- 应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
- 说明
-
Antigen size: 659 AA
- 限制
- 仅限研究用
-
- by
- Shaw Lab, Department of Biochemistry, University of Utah
- No.
- #100008
- 日期
- 2015.09.12
- 抗原
- RHOT1
- Lot Number
- Method validated
- Western Blotting
- Positive Control
Purified recombinant Miro1
Isolated mitochondria from immortalized Miro1 KO MEFs expressing Myc-Miro1
- Negative Control
Purified recombinant Miro2
Isolated mitochondria from immortalized Miro1 WT MEFs
Isolated mitochondria from immortalized Miro1 KO MEFs expressing Myc-Miro2
- Notes
Passed. ABIN405704 specifically recognizes Miro1 without cross-reaction with Miro2.
- Primary Antibody
- ABIN405704
- Secondary Antibody
- Goat-anti-rabbit IgG HRP conjugated (Sigma, A0545-1M22)
- Full Protocol
- Transfect immortalized Miro1-/- MEFs with Myc-Miro1-V1 or Myc-Miro2-V1 as described in PMID 25136135.
- Isolate mitochondria from immortalized Miro1+/+, Miro1-/-, Miro1-/- + Myc-Miro1 and Miro1-/- + Myc-Miro2 MEFs.
- Determine protein concentration and snap freeze mitochondria in liquid nitrogen.
- Thaw 30 µg mitochondria per lane on ice.
- Thaw 100 ng recombinant Miro1 and Miro2 per lane on ice.
- Add 2x Laemmli buffer and 2% BME to samples and denature for 5 min at 95 °C.
- Separte 30 µg/lane mitochondria and 100 ng/lane recombinant protein on a 12.5% SDS-PAGE for 3 h at 40mA/180V.
- Semi-dry transfer to PVDF membrane for 2 h at 250 mA/15 V.
- Block the membrane with TBST + 5% non-fat dry milk for 1 h at RT.
- Incubate with primary
- rabbit anti-RHOT1 antibody (antibodies-online, ABIN405704) diluted 1:1000 in TBST + 3% BSA overnight at 4°C.
- rabbit anti-Myc1 antibody diluted 1:1000 in TBST + 3% BSA overnight at 4°C.
- rabbit anti-Cyt c antibody diluted 1:1000 in TBST + 3% BSA overnight at 4°C.
- Wash membrane 3x for 5 min with TBST buffer.
- Incubate with secondar goat anti-rabbit IgG HRP antibody (Sigma, A0545-1M2) diluted 1:2000 in TBST + 5% non-fat dry milk for 1 h at RT.
- Wash membrane 3x for 10 min with TBST buffer.
- Reveal protein bands on a Bio-Rad imaging system using homemade ECL prepared according to PMID 17141265. <
- Experimental Notes
生效 #100008 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![]()
Full Methods -
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
-
- 抗原
- RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))
- 别名
- RHOT1 (RHOT1 产品)
- 别名
- rhot1 antibody, zgc:55581 antibody, zgc:77063 antibody, wu:fd14e11 antibody, wu:fi14a05 antibody, arht2 antibody, miro-2 antibody, rasl antibody, ARHT1 antibody, MIRO-1 antibody, MIRO1 antibody, 2210403N23Rik antibody, AA415293 antibody, AF244542 antibody, AI834919 antibody, Arht1 antibody, C430039G08Rik antibody, Miro1 antibody, miro1 antibody, si:dkeyp-97g3.6 antibody, ras homolog family member T1a antibody, ras homolog family member T2 antibody, ras homolog family member T1 antibody, ras homolog family member T1 L homeolog antibody, rhot1a antibody, rhot2 antibody, RHOT1 antibody, rhot1 antibody, rhot1.L antibody, Rhot1 antibody, rhot1b antibody
- 背景
-
RHOT1 is mitochondrial GTPase involved in mitochondrial trafficking. It is probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
Alias Symbols: ARHT1, FLJ11040, FLJ12633, MIRO-1, MIRO1
Protein Interaction Partner: PCSK9, ERLIN2, PARK2, ILK, UBC, CFTR, PINK1, UBD, IRAK1, AMFR,
Protein Size: 659 - 分子量
- 75 kDa
- 基因ID
- 55288
- NCBI登录号
- NM_001033566, NP_001028738
- UniProt
- Q8IXI2
-
