JNK 抗体 (pThr183, pTyr185)
Quick Overview for JNK 抗体 (pThr183, pTyr185) (ABIN401562)
抗原
See all JNK (MAPK8) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr183, pTyr185
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特异性
- This antibody detects endogenous levels of JNK1/JNK2 only when phosphorylated at Thr183/Tyr185.
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纯化方法
- Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- The antiserum was produced against synthesized phosphopeptide derived from human JNK1/JNK2 around the phosphorylation site of Thr183/Tyr185 (M-M-Tp-P-YP-V-V).
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anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 245-345) antibody
MAPK8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (pThr183), (pTyr185) antibodyMAPK8 适用: 人, 小鼠, 大鼠, 犬, 兔 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 138-370) antibodyMAPK8 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-384) antibodyMAPK8 适用: 人 WB, ELISA, IF, PLA, RNAi 宿主: 小鼠 Monoclonal 2F11 unconjugated
anti-Mitogen-Activated Protein Kinase 8 (MAPK8) antibodyMAPK8 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 201-300) antibodyMAPK8 适用: 人, 小鼠, 大鼠, 小鸡, 犬 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 145-419) antibodyMAPK8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 1-427) antibodyMAPK8 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 318-427) antibodyMAPK8 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 4A11 unconjugated
anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 151-250) antibodyMAPK8 适用: 人, 小鼠, 大鼠, 小鸡, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at-20 °C.
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- JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
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别名
- MAPK8 / JNK1
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背景
- JNK (c-Jun N-terminal Kinase), also referred to as Stress Activated Protein Kinase (SAPK), is one of the main mitogen-activated protein kinases (MAPKs) in mammals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2. JNK is activated by a variety of cellular signals including growth factors, inflammatory cytokines, and environmental stress. The JNK/SAPK signaling pathway involves sequential activation of MAPK kinase kinase (MEKK1), MAPK kinase 4 (MKK4) or MKK7, SAPK/JNK, and c-Jun. Full activation of JNK requires phosphorylation of a threonine and a tyrosine residue in the motif Thr-Pro-Tyr. MKK7 and MKK4 phosphorylate JNK at threonine 183 and tyrosine 185, respectively. The JNK pathway functions to modulate cell cycle, apoptotic and transcriptional responses to stress.Synonyms: JNK-46, JNK-55, JNK1/JNK2, MAPK8, MAPK9, PRKM8, PRKM9, SAPK1, c-Jun N-terminal kinase 1, c-Jun N-terminal kinase 2
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基因ID
- 5599
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NCBI登录号
- NP_002741
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UniProt
- P45983
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途径
- MAPK Pathway, WNT signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins
抗原
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