HHV8 抗体 (C-Term)
HHV8
适用: 人
WB, EIA
宿主: 兔
Polyclonal
unconjugated
1 image
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- 抗原 See all HHV8 products
- HHV8 (Herpes Virus Type 8 (HHV8))
- 抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HHV8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 纯化方法
- Affinity chromatography
- 免疫原
- Synthetic peptide corresponding to a region near the C-terminal of human KSHV ORF57 protein
- 亚型
- IgG
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anti-Herpes Virus Type 8 (HHV8) antibody
HHV8 适用: 人 IHC (p) 宿主: 大鼠 Monoclonal LN53 unconjugated
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- 应用备注
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ELISA: 1/100,000 - 1/170,000. Western Blot: 1/5,000 - 1/8,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.55 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
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- 抗原
- HHV8 (Herpes Virus Type 8 (HHV8))
- 别名
- Herpes Virus Type 8 / HHV8 (HHV8 产品)
- 物质类
- Virus
- 背景
- ORF57 (also known as MTA), one of the earliest Kaposi's sarcoma-associated herpesvirus (KSHV) regulatory proteins to be expressed, is essential for virus lytic replication. A counterpart is present in every herpesvirus sequenced, indicating the importance of this signature viral protein, and those examined act post-transcriptionally, affecting RNA splicing and transport. KSHV ORF57 is capable of establishing both lytic and latent replication cycles. In KS, the virus localizes to tumor progenitor endothelial cells, most of which are latently infected. In cell culture, KSHV replication is generally studied using B-cell lines, such as BCBL-1, generated from primary effusion lymphoma material. Most BCBL-1 cells are latently infected, although there is some spontaneous virus reactivation. Addition of chemical inducers such as sodium n-butyrate, 12-O-tetradecanoylphorbol-13-acetate (TPA), and valproic acid (VA) to these cells efficiently induces the lytic cycle and produces virions. KSHV ORF57 protein predominantly localizes to the nucleus and can shuttle between the nucleus and cytoplasm. Most HSV-1 genes are unspliced, by contrast, ORF57 is spliced gene, the protein is 455 amino acids in length and 50 kDa in size.Synonyms: HHV-8, KHSV
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