This antibody reacts with Human THRA protein. A BLAST analysis was used to suggest cross-reactivity with THRA from Mouse, Human and Rat based on a 100 % homology with the immunizing sequence. Cross-reactivity with THRA from other sources has not been determined.
纯化方法
Immunoaffinity Chromatography
免疫原
Synthetic peptide corresponding to a region near the amino terminus of Human THRA isoform 1 protein . Genename: THRA
ELISA: 1/650,000. Western Blot: 1/1,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.89 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Thyroid hormone receptor alpha is a nuclear hormone receptor with high affinity for the hormone triiodothyronine. THRA is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having a certain extent of redundancy, may mediate different functions of thyroid hormone. THRA interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase in transcription of target genes. THRA is localized within the nucleus and has been found to exist as 4 isoforms originating from alternative splicing variants. This antibody recognizes THRA isoform 1. Isoform 1 has a distinct C-terminus compared to isoform 2.Synonyms: C-erbA-alpha, EAR-7, EAR7, NR1A1, Nuclear receptor subfamily 1 group A member 1, THRA1, THRA2, Thyroid hormone receptor alpha, c-erbA-1