MYL6 抗体 (AA 12-27)
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- 抗原 See all MYL6 抗体
- MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))
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抗原表位
- AA 12-27
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MYL6 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is directed against the regulatory light chain of smooth and nonmuscle Myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
- 纯化方法
- Immunoaffinity Chromatography.
- 免疫原
- Synthetic peptide corresponding to amino acids 12-27 of Human Myosin light chain protein.
- 亚型
- IgG
- Top Product
- Discover our top product MYL6 Primary Antibody
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anti-Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6) antibody
MYL6 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6) (AA 1-100) antibodyMYL6 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D6 unconjugated
anti-Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6) (AA 72-121) antibodyMYL6 适用: 人, 小鼠, 大鼠, Cow, 绵羊, 犬, 豚鼠, 马, 兔, Pig, 斑马鱼, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6) (AA 3-151) antibodyMYL6 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6) (AA 1-100) antibodyMYL6 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6) (Internal Region) antibodyMYL6 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6) (pSer19), (pSer20) antibodyMYL6 适用: 人 WB, ELISA, IHC (p), IP 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6) (AA 4-146) antibodyMYL6 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6) (pSer30), (pTyr29) antibodyMYL6 适用: 小鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6) (C-Term) antibodyMYL6 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Tested in ELISA (1/5000-1/20000) and Western Blot (1/500-1/2000). Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation (1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.71 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))
- 别名
- MYL6 (MYL6 产品)
- 别名
- ESMLC antibody, LC17 antibody, LC17-GI antibody, LC17-NM antibody, LC17A antibody, LC17B antibody, MLC-3 antibody, MLC1SM antibody, MLC3NM antibody, MLC3SM antibody, zgc:103624 antibody, MLC3nm antibody, Myln antibody, myosin light chain 6 antibody, myosin, light chain 6, alkali, smooth muscle and non-muscle antibody, myosin light chain 6 L homeolog antibody, myosin, light polypeptide 6, alkali, smooth muscle and non-muscle antibody, MYL6 antibody, Myl6 antibody, myl6 antibody, myl6.L antibody
- 背景
- Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, mouse and rat have almost identical sequences.Synonyms: 17 kDa myosin light chain, LC17, MLC-3, Myosin light chain 3, Myosin light chain alkali 3, Myosin light polypeptide 6, Smooth muscle and nonmuscle myosin light chain alkali 6
- 基因ID
- 4637
- NCBI登录号
- NP_066299
- UniProt
- P60660
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