ECT2 抗体 (pThr790)
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- 抗原 See all ECT2 抗体
- ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))
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抗原表位
- AA 785-795, pThr790
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适用
- 人, 小鼠, 大鼠, 犬, 斑马鱼, 小鸡, Chimpanzee
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ECT2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is directed against the phosphorylated form of ECT2 protein at the pT790 residue.
- 交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Zebrafish, Chimpanzee, Chicken, Canine.
Species reactivity (tested):Human. - 纯化方法
- Affinity chromatography
- 免疫原
- Synthetic peptide corresponding to amino acids 785-795 of human ECT2 protein
- 亚型
- IgG
- Top Product
- Discover our top product ECT2 Primary Antibody
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anti-Epithelial Cell Transforming Sequence 2 Oncogene (ECT2) (AA 283-453) antibody
ECT2 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Transforming Sequence 2 Oncogene (ECT2) (N-Term) antibodyVerified ECT2 适用: 人 ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-Epithelial Cell Transforming Sequence 2 Oncogene (ECT2) (AA 453-647) antibodyECT2 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Transforming Sequence 2 Oncogene (ECT2) (AA 166-348) antibodyECT2 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Transforming Sequence 2 Oncogene (ECT2) (AA 781-883) antibodyECT2 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Transforming Sequence 2 Oncogene (ECT2) (AA 46-145) antibodyECT2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5D4 unconjugated
anti-Epithelial Cell Transforming Sequence 2 Oncogene (ECT2) (AA 200-460) antibodyECT2 适用: 人 WB 宿主: 小鼠 Monoclonal 1691CT516-3-3 unconjugated
anti-Epithelial Cell Transforming Sequence 2 Oncogene (ECT2) antibodyECT2 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Transforming Sequence 2 Oncogene (ECT2) (AA 580-629) antibodyECT2 适用: 人, 小鼠, 大鼠, 犬, 猴, 豚鼠, 兔, 马, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Transforming Sequence 2 Oncogene (ECT2) (AA 551-600) antibodyECT2 适用: 人, 小鼠, 大鼠, 犬, 斑马鱼, 猴, 豚鼠, 兔, Cow WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/10,000 - 1/40,000. Western Blot: 1/1,000 - 1/4,000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.31 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
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- 抗原
- ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))
- 别名
- ECT2 (ECT2 产品)
- 别名
- ECT2 antibody, arhgef31 antibody, xect2 antibody, AI528536 antibody, mKIAA4037 antibody, ARHGEF31 antibody, ect2 antibody, epithelial cell transforming 2 antibody, ect2 oncogene antibody, epithelial cell transforming 2 S homeolog antibody, ECT2 antibody, ect2 antibody, Ect2 antibody, ect2.S antibody
- 背景
- ECT2, also known as epithelial cell transforming sequence 2 oncogene, was originally isolated as a transforming gene from epithelial cells. ECT2 catalyzes guanine nucleotide exchange on the small GTPases, RhoA, Rac1, and Cdc42. ECT2 may be phosphorylated during G2 and M phases, and phosphorylation may be required for its exchange activity. Unlike other known guanine nucleotide exchange factors for Rho GTPases, ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis. Expression of dominant-negative ECT2 or microinjection of affinity-purified anti-ECT2 antibody into interphase cells strongly inhibits cytokinesis. These results suggest that ECT2 is an important link between the cell cycle machinery and Rho signaling pathways involved in the regulation of cell division. Phosphorylation at T790 or S375 significantly affects the catalytic activity of ECT2.Synonyms: Epithelial cell-transforming sequence 2 oncogene
- 基因ID
- 1894
- NCBI登录号
- NP_060568
- UniProt
- Q9H8V3
- 途径
- Neurotrophin Signaling Pathway, Cell-Cell Junction Organization
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