NOXO1 抗体 (AA 238-252)
(1 reference)Quick Overview for NOXO1 抗体 (AA 238-252) (ABIN401322)
抗原
See all NOXO1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 238-252
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特异性
- This antibody is directed against NOXO1 protein. Expect a band approximately 50 kDa in size corresponding to NOXO1 protein by western blotting in the appropriate cell lysate or extract. A BLAST analysis was used to suggest cross reactivity with NOXO1 protein from Human and Chimpanzee (100 % homology). Expect reactivity with alpha, delta and gamma isoforms of NOXO1. Also expect partial reactivity against NOXO1 homologues from Dog and Guinea Pig (87 %), as well as Rat (75 %) and Mouse (68 %). Reactivity against homologues from other sources is not known.
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纯化方法
- Immunoaffinity Chromatography.
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免疫原
- Recombinant protein corresponding to amino acids 238-252 of Human NOXO1 protein
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亚型
- IgG
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anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 304-376) antibody
NOXO1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (C-Term) antibodyVerified NOXO1 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 1-100) antibodyNOXO1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (C-Term) antibodyNOXO1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 121-370) antibodyNOXO1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (C-Term) antibodyNOXO1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 238-252) antibodyNOXO1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- This affinity purified antibody is tested for use in ELISA, Immunohistochemistry andWestern blot. Recommended Dilutions: ELISA: 1/2,0000-1/100,000.
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限制
- 仅限研究用
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浓度
- 1.17 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7. containing 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at-20 °C. Dilute only prior to immediate use.
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: "HSP90 inhibition by 17-DMAG attenuates oxidative stress in experimental atherosclerosis." in: Cardiovascular research, Vol. 95, Issue 1, pp. 116-23, (2012) (PubMed).
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: "HSP90 inhibition by 17-DMAG attenuates oxidative stress in experimental atherosclerosis." in: Cardiovascular research, Vol. 95, Issue 1, pp. 116-23, (2012) (PubMed).
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- NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))
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别名
- NOXO1
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背景
- The enzymes NADPH oxidase (NOX) and dual oxidase (DUOX) generate ROS in a regulated manner, producing reactive oxygen in various cells and tissues in response to growth factors, cytokines and calcium signals. The oxidase consists of the catalytic subunit gp91phox (otherwise known as NOX2), together with the regulatory subunits p22phox, p47phox, p40phox, p67phox and the small GTPase RAC. The enzyme activity of gp91phox is regulated by the assembly of these regulatory subunits with gp91phox to form an active complex. In 1999, the first of the NOX homologues of gp91phox was described as NOX1. The enzyme was cloned from a colon epithelial cell complementary DNA library. When expressed in cells, NOX1 generated low amounts of ROS, but high-level ROS production by NOX1 was subsequently achieved by co-expression with novel regulatory subunits (described later). Subsequently, NOX3 and NOX4 were cloned, and the latter was shown to generate high levels of ROS when expressed in cells. NOX organizer 1 (NOXO1) is a homologue of p47phox and has an almost identical domain organization, except that it lacks the auto-inhibitory region. NOX activator 1 (NOXA1) is a homologue of p67phox and similarly shares the same domain organization, except that it lacks one of the two SH3 domains that are present in p67phox. Co-transfection of NOX1, NOXO1 and NOXA1 results in marked ROS generation. Similar to p47phox, NOXO1 binds to p22phox, which is required for NOX1-dependent activity. NOXA1 has a well-conserved activation domain, implying a conserved mechanism for regulating the activity of the target NOX enzyme.Synonyms: NADPH oxidase organizer 1, P41NOX, SH3PXD5
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基因ID
- 124056
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NCBI登录号
- NP_653204
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UniProt
- Q8NFA2
抗原
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