This antibody was assayed by Immunoblot and found to be reactive against Frequenin at a dilution of 1/5000 followed by reaction with Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG [H&L] Cat.-No R1364HRP. This antibody is suitable for the detection by Immunoblot of Human, Mouse and Rat Frequenin. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
纯化方法
Immunoaffinity Chromatography using Frequenin (recombinant) coupled to agarose beads.
免疫原
Frequenin (recombinant from Mouse with extensive post-translational modifications)
ELISA. Western Blot: 1/5000. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
2.0 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Frequenin is a Ca2+ binding protein implicated in the regulation of neurotransmitter release at the neuromuscular junction. Frequenin amino acid sequences from different species appear closely related. In mouse a single 4.2 kbp mRNA is produced yielding a protein of 22 kDa when assayed by SDSPAGE under reducing conditions. Frequenin may colocalize with the dendritic marker MAP-2 and is widely distributed in the brain, spinal cord and dorsal root ganglia. Reactivity with avian and invertebrate frequenin is likely. In Drosophila frequenin is related to recoverin and visinin and functions like Ca2+-sensitive guanylyl cyclase activator.Synonyms: FLUP, FREQ, NCS-1, Neuronal calcium sensor 1