GPC1 抗体 (Internal Region)
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- 抗原 See all GPC1 抗体
- GPC1 (Glypican 1 (GPC1))
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抗原表位
- Internal Region
- 适用
- 人, 小鼠, 大鼠, 犬, Macaque, Opossum
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GPC1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is specific for Glypican-1 protein.
- 交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Macaque, Canine, Cattle, Opossum.
Species reactivity (tested):Human. - 纯化方法
- Affinity Chromatography on Protein A
- 免疫原
- Synthetic peptide corresponding to an internal region of Human Glypican-1 protein.
- 亚型
- IgG
- Top Product
- Discover our top product GPC1 Primary Antibody
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anti-Glypican 1 (GPC1) antibody
KO Validated GPC1 适用: 人 WB, ELISA, IP, ICC, IF, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 155-292) antibodyGPC1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 342-530) antibodyGPC1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 24-131) antibodyGPC1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 12-41), (N-Term) antibodyGPC1 适用: 人 WB, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 301-400) antibodyGPC1 适用: 人 ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 114-277) antibodyGPC1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 343-545) antibodyGPC1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (Internal Region) antibodyVerified GPC1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glypican 1 (GPC1) (AA 290-420) antibodyGPC1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1: 2,500 - 1: 3000. Western Blot: 1: 800 - 1: 1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 2.1 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Dilute only prior to immediate use. Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- GPC1 (Glypican 1 (GPC1))
- 别名
- Glypican-1 / GPC1 (GPC1 产品)
- 别名
- gpc1 antibody, zgc:63947 antibody, GPC1 antibody, Glypican-1 antibody, glypican antibody, AI462976 antibody, HSPG M12 antibody, HSPGM12 antibody, glypican 1b antibody, glypican 1 antibody, gpc1b antibody, GPC1 antibody, gpc1 antibody, Gpc1 antibody
- 背景
- Glypican-1 (also known as GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary from 17 % to 63 % identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican-1 has been shown to interact with SLIT2.
- 基因ID
- 2817
- NCBI登录号
- NP_002072
- UniProt
- P35052
- 途径
- Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation
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