(6 references)-
- 抗原 See all HRP products
- HRP (Horseradish Peroxidase (HRP))
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适用
- Horseradish
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HRP antibody is un-conjugated
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应用范围
- ELISA
- 特异性
- Rabbit Anti-HRP Polyclonal Antibody is specific to peroxidase (horseradish).
- 纯化方法
- Protein G chromatography
- 免疫原
- Purified peroxidase from horseradish
- 亚型
- IgG
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anti-Horseradish Peroxidase (HRP) antibody
HRP 适用: Horseradish ELISA, WB, ICC 宿主: 小鼠 Monoclonal HP-03 unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Other applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.5 mg/mL
- 缓冲液
- PBS, pH 7.4, containing 30 % glycerol, and 0.02 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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: "A new insight to explore the regulation between S-nitrosylation and N-glycosylation." in: Plant direct, Vol. 3, Issue 2, pp. e00110, (2019) (PubMed).
: "Plasma Cytochrome c Detection Using a Highly Sensitive Electrochemiluminescence Enzyme-Linked Immunosorbent Assay." in: Biomarker insights, Vol. 12, pp. 1177271917746972, (2017) (PubMed).
: "C-type lectin-like molecule-1 (CLL1)-targeted TRAIL augments the tumoricidal activity of granulocytes and potentiates therapeutic antibody-dependent cell-mediated cytotoxicity." in: mAbs, Vol. 7, Issue 2, pp. 321-30, (2015) (PubMed).
: "OsMOGS is required for N-glycan formation and auxin-mediated root development in rice (Oryza sativa L.)." in: The Plant journal : for cell and molecular biology, Vol. 78, Issue 4, pp. 632-45, (2014) (PubMed).
: "Use of MSG1 protein in a novel blocking ELISA for the detection of Mycoplasma suis infection." in: Veterinary journal (London, England : 1997), Vol. 193, Issue 2, pp. 535-8, (2012) (PubMed).
: "Sequence-dependent prion protein misfolding and neurotoxicity." in: The Journal of biological chemistry, Vol. 285, Issue 47, pp. 36897-908, (2010) (PubMed).
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: "A new insight to explore the regulation between S-nitrosylation and N-glycosylation." in: Plant direct, Vol. 3, Issue 2, pp. e00110, (2019) (PubMed).
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- 抗原
- HRP (Horseradish Peroxidase (HRP))
- 别名
- HRP (HRP 产品)
- 背景
- Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator enzyme in reactions in which peroxide is produced, such as in conjunction with glucose oxidase in the evaluation of glucose in biological fluids. It also can be used as an enzyme label, such as in ELISA systems and peroxidase-anti-peroxidase complexes in immunohistochemistry. Rabbit Anti-HRP Polyclonal Antibody is developed in rabbit hosts using purified peroxidase from horseradish as the immunogen.Rabbit Anti-HRP Polyclonal Antibody is purified from rabbit antiserum by Protein G chromatography and is supplied as a 200 µg aliquot at a concentration of 0.5 mg/ml.
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