APEX1 抗体
-
- 抗原 See all APEX1 抗体
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This APEX1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- IHC-plus™
- 特异性
- Specific to the human APE/ref-1protein. Competitively inhibited from recognizing the APE/ref-1 antigen in tissues using APE/ref-1 protein. Inhibits repair activity of ape protein.
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Affinity purified human Apurinic/Apyrimidinic Endonuclease (APE/ref-1) fusion protein.
Type of Immunogen: Fusion protein - 亚型
- IgG
- Top Product
- Discover our top product APEX1 Primary Antibody
-
-
- 应用备注
-
Approved: IHC, IHC-Fr, IHC-P (1:250), WB (1:500)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:250. Western Blot: 1:500 (ECL). Detects a single band at 37kD. Diluted antibody can be reused 1-3 times in Western Blot analysis. Apoptosis: Can be used following the apoptosis (TUNEL) procedure using the Boehringer-Mannheim TUNEL assay kit. Antibody staining should be performed AFTER the TUNEL assay. Positive control: HeLa whole cell extract. - 说明
-
Target Species of Antibody: Human
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.2, 0.05 % sodium azide before the addition of glycerol to 40 %.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
- 抗原
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
- 别名
- APEX1 / APE1 (APEX1 产品)
- 别名
- apex1 antibody, MGC76280 antibody, MGC79645 antibody, MGC78928 antibody, APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, Apex antibody, REF-1 antibody, BAP1 antibody, apex1a antibody, hm:zehn1532 antibody, wu:fc13d11 antibody, zgc:66204 antibody, Ref-1 antibody, apurinic/apyrimidinic endodeoxyribonuclease 1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog antibody, DNA-(apurinic or apyrimidinic site) lyase antibody, apurinic/apyrimidinic endonuclease 1 antibody, APEX1 antibody, apex1 antibody, apex1.L antibody, CNG03640 antibody, CNH01280 antibody, nth antibody, Spirs_4119 antibody, Palpr_0103 antibody, Apex1 antibody
- 背景
-
Name/Gene ID: APEX1
Synonyms: APEX1, AP endonuclease class I, AP lyase, APE, APE-1, APEN, APE1, APEX nuclease, Redox factor-1, AP endonuclease 1, APEX, APX, Protein REF-1, REF-1, REF1 - 基因ID
- 328
- UniProt
- P27695
- 途径
- DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
-