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APEX1 抗体

APEX1 适用: 人 WB, IHC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN396973
发货至: 中国
  • 抗原 See all APEX1 抗体
    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
    适用
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    宿主
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    克隆类型
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    多克隆
    标记
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    This APEX1 antibody is un-conjugated
    应用范围
    • 80
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    品牌
    IHC-plus™
    特异性
    Specific to the human APE/ref-1protein. Competitively inhibited from recognizing the APE/ref-1 antigen in tissues using APE/ref-1 protein. Inhibits repair activity of ape protein.
    纯化方法
    Immunoaffinity purified
    免疫原
    Affinity purified human Apurinic/Apyrimidinic Endonuclease (APE/ref-1) fusion protein.

    Type of Immunogen: Fusion protein
    亚型
    IgG
    Top Product
    Discover our top product APEX1 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-Fr, IHC-P (1:250), WB (1:500)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:250. Western Blot: 1:500 (ECL). Detects a single band at 37kD. Diluted antibody can be reused 1-3 times in Western Blot analysis. Apoptosis: Can be used following the apoptosis (TUNEL) procedure using the Boehringer-Mannheim TUNEL assay kit. Antibody staining should be performed AFTER the TUNEL assay. Positive control: HeLa whole cell extract.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS,  pH 7.2, 0.05 % sodium azide before the addition of glycerol to 40 %.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原
    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
    别名
    APEX1 / APE1 (APEX1 产品)
    别名
    apex1 antibody, MGC76280 antibody, MGC79645 antibody, MGC78928 antibody, APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, Apex antibody, REF-1 antibody, BAP1 antibody, apex1a antibody, hm:zehn1532 antibody, wu:fc13d11 antibody, zgc:66204 antibody, Ref-1 antibody, apurinic/apyrimidinic endodeoxyribonuclease 1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog antibody, DNA-(apurinic or apyrimidinic site) lyase antibody, apurinic/apyrimidinic endonuclease 1 antibody, APEX1 antibody, apex1 antibody, apex1.L antibody, CNG03640 antibody, CNH01280 antibody, nth antibody, Spirs_4119 antibody, Palpr_0103 antibody, Apex1 antibody
    背景
    Name/Gene ID: APEX1

    Synonyms: APEX1, AP endonuclease class I, AP lyase, APE, APE-1, APEN, APE1, APEX nuclease, Redox factor-1, AP endonuclease 1, APEX, APX, Protein REF-1, REF-1, REF1
    基因ID
    328
    UniProt
    P27695
    途径
    DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
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