CENPF 抗体 (AA 1759-2093)
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北京 101111
Quick Overview for CENPF 抗体 (AA 1759-2093) (ABIN396951)
抗原
See all CENPF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1759-2093
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特异性
- Recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
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纯化方法
- Protein G purified
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免疫原
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GST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin.
Type of Immunogen: Fusion protein -
亚型
- IgG2a kappa
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anti-Centromere Protein F (CENPF) (C-Term) antibody
CENPF 适用: 人 WB, IHC (p), IF, ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (AA 436-637) antibodyCENPF 适用: 人 ELISA, WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (C-Term) antibodyCENPF 适用: 人, 小鼠 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (AA 551-650) antibodyCENPF 适用: 人 ELISA, WB, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein F (CENPF) (C-Term) antibodyVerified CENPF 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Approved: IHC, IHC-Fr (10 μg/mL), IHC-P (10 μg/mL), IP (1 - 10 μg/mL), WB (1 - 10 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-10 μg/mL. Immunoprecipitation: 1-10 μg/mL. Immunohistochemistry: 10 μg/mL with frozen or paraffin-embedded sections. Tested in IHC using formalin-fixed paraffin-embedded human tissues, HIER antigen retrieval using sodium citrate buffer at pH 6.0. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -70°C, avoid freeze/thaw cycles.
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- CENPF (Centromere Protein F (CENPF))
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别名
- CENPF / CENP-F
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背景
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Name/Gene ID: CENPF
Synonyms: CENPF, AH antigen, Centromere protein F, CENF, CENP-F, Kinetochore protein CENPF, Mitosin, CENP-F kinetochore protein, Hcp-1, PRO1779 -
基因ID
- 1063
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UniProt
- P49454
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途径
- Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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