Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
预测反应
C, Pig
纯化方法
This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This NRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the N-terminal region of human NRAS.
NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).