This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This IL3R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 744-773 amino acids from the C-terminal region of human IL3R.
Purified rabbit polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.