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ATG5 抗体 (N-Term)

Name: ATG5 抗体 (N-Term)
SKU: ABIN388518
Availability: OutOfStock

ATG5 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7466 unconjugated

All lanes : Anti-hG5L-D3 at 1:1000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. (ATG5 抗体 (N-Term))

Immunohistochemical analysis of paraffin-embedded human liver tissue using (ABIN388518 and ABIN2849631) performed on the Leica® BOND RXm. (ATG5 抗体 (N-Term))

IHC (p)

Fluorescent image of cells stained with ATG5 (N-term) antibody. (ATG5 抗体 (N-Term))

3 images

(23 references)

产品编号 ABIN388518

发货至: 中国

Datasheet as PDF

抗原 See all ATG5 抗体

ATG5 (ATG5 Autophagy Related 5 (ATG5))
抗原表位
16

12

7

6

6

5

5

5

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1
AA 1-30, N-Term
适用
102

44

36

7

6

6

6

5

4

4

4

3

3

2

1
人
宿主
77

22

4

1
兔
Compatible Secondaries
克隆类型
76

28
多克隆
标记
57

8

7

6

6

6

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ATG5 antibody is un-conjugated
应用范围
79

54

36

18

17

17

14

13

10

10

6

3

3

3

2

2

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

预测反应

Zf, B, M, Pig, Rat

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ATG5.

克隆位点

RB7466

亚型

Ig Fraction

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anti-ATG5 Autophagy Related 5 (ATG5) (AA 101-200) antibody
ATG5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-275) antibody
ATG5 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 209-238), (C-Term) antibody
ATG5 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7547 unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 144-275) antibody
ATG5 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 3C7A8 unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-119) antibody
ATG5 适用: 人 WB, ELISA, IHC, Coat, StM 宿主: 小鼠 Monoclonal ATG5-2101 unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 176-275) antibody
ATG5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G11 unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-30) antibody
ATG5 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-30), (N-Term) antibody
ATG5 适用: 人, 小鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (C-Term) antibody
ATG5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-50) antibody
ATG5 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, Pig, 非洲爪蟾, 灵长类 WB, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:200. WB: 1:1000. IHC-P-Leica: 1:500

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Kessel, Reiners: "Effects of Combined Lysosomal and Mitochondrial Photodamage in a Non-small-Cell Lung Cancer Cell Line: The Role of Paraptosis." in: Photochemistry and photobiology, Vol. 93, Issue 6, pp. 1502-1508, (2018) (PubMed).

Kessel, Evans: "Promotion of Proapoptotic Signals by Lysosomal Photodamage: Mechanistic Aspects and Influence of Autophagy." in: Photochemistry and photobiology, Vol. 92, Issue 4, pp. 620-3, (2017) (PubMed).

Mathai, Meijer, Simonsen: "Studying Autophagy in Zebrafish." in: Cells, Vol. 6, Issue 3, (2017) (PubMed).

Lim, Zare, Puertollano, Raben: "Atg5flox-Derived Autophagy-Deficient Model of Pompe Disease: Does It Tell the Whole Story?" in: Molecular therapy. Methods & clinical development, Vol. 7, pp. 11-14, (2017) (PubMed).

Sasaki, Yamashita, Shin: "Autophagy in spinal motor neurons of conditional ADAR2-knockout mice: An implication for a role of calcium in increased autophagy flux in ALS." in: Neuroscience letters, Vol. 598, pp. 79-84, (2015) (PubMed).

Wang, Qi, Wang, Zhu, Wang, Jin, Tan, Huang, Xu, Li, Kuang, Tang, Du, Chen, Chen: "FGFR3/Fibroblast Growth Factor Receptor 3 Inhibits Autophagy through Decreasing the ATG12-ATG5 Conjugate, Leading to the Delay of Cartilage Development in Achondroplasia." in: Autophagy, (2015) (PubMed).

Chen, Huang, Yuan, Cheng, Zhou: "Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells." in: Molecular biology and evolution, Vol. 31, Issue 8, pp. 2194-211, (2014) (PubMed).

Xin, Xiao, Ma, He, Yao, Liu et al.: "Coxsackievirus B3 induces crosstalk between autophagy and apoptosis to benefit its release after replicating in autophagosomes through a mechanism involving caspase cleavage of autophagy-related ..." in: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, Vol. 26, pp. 95-102, (2014) (PubMed).

Cha, Hwang, Kim, Choi, Oh, Roh: "Autophagy induced by tumor necrosis factor ? mediates intrinsic apoptosis in trophoblastic cells." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 21, Issue 5, pp. 612-22, (2014) (PubMed).

Chinskey, Zheng, Zacks: "Control of photoreceptor autophagy after retinal detachment: the switch from survival to death." in: Investigative ophthalmology & visual science, Vol. 55, Issue 2, pp. 688-95, (2014) (PubMed).

Schober, Parlato, Huber, Kinscherf, Hartleben, Huber, Schütz, Unsicker: "Cell loss and autophagy in the extra-adrenal chromaffin organ of Zuckerkandl are regulated by glucocorticoid signalling." in: Journal of neuroendocrinology, Vol. 25, Issue 1, pp. 34-47, (2013) (PubMed).

Keil, Höcker, Schuster, Essmann, Ueffing, Hoffman, Liebermann, Pfeffer, Schulze-Osthoff, Schmitz: "Phosphorylation of Atg5 by the Gadd45?-MEKK4-p38 pathway inhibits autophagy." in: Cell death and differentiation, Vol. 20, Issue 2, pp. 321-32, (2013) (PubMed).

Khaldoun, Emond-Boisjoly, Chateau, Carrière, Lacasa, Rousset, Demignot, Morel: "Autophagosomes contribute to intracellular lipid distribution in enterocytes." in: Molecular biology of the cell, Vol. 25, Issue 1, pp. 118-32, (2013) (PubMed).

Liu, He, von Rütte, Yousefi, Hunger, Simon: "Down-regulation of autophagy-related protein 5 (ATG5) contributes to the pathogenesis of early-stage cutaneous melanoma." in: Science translational medicine, Vol. 5, Issue 202, pp. 202ra123, (2013) (PubMed).

Chiu, Chen, Ho, Wang: "Arsenic trioxide enhances the radiation sensitivity of androgen-dependent and -independent human prostate cancer cells." in: PLoS ONE, Vol. 7, Issue 2, pp. e31579, (2012) (PubMed).

Hu, Zhang, Zhang: "Expression pattern and functions of autophagy-related gene atg5 in zebrafish organogenesis." in: Autophagy, Vol. 7, Issue 12, pp. 1514-27, (2012) (PubMed).

Lépine, Allegood, Edmonds, Milstien, Spiegel: "Autophagy induced by deficiency of sphingosine-1-phosphate phosphohydrolase 1 is switched to apoptosis by calpain-mediated autophagy-related gene 5 (Atg5) cleavage." in: The Journal of biological chemistry, Vol. 286, Issue 52, pp. 44380-90, (2011) (PubMed).

Chiu, Ho, Wang: "Arsenic trioxide induces autophagy and apoptosis in human glioma cells in vitro and in vivo through downregulation of survivin." in: Journal of molecular medicine (Berlin, Germany), Vol. 89, Issue 9, pp. 927-41, (2011) (PubMed).

Lépine, Allegood, Park, Dent, Milstien, Spiegel: "Sphingosine-1-phosphate phosphohydrolase-1 regulates ER stress-induced autophagy." in: Cell death and differentiation, Vol. 18, Issue 2, pp. 350-61, (2011) (PubMed).

Hnasko, Lin, McGarvey, Stanker: "A rapid method to improve protein detection by indirect ELISA." in: Biochemical and biophysical research communications, Vol. 410, Issue 4, pp. 726-31, (2011) (PubMed).

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抗原

ATG5 (ATG5 Autophagy Related 5 (ATG5))

别名

ATG5 (ATG5 产品)

别名

APG5 antibody, APG5-LIKE antibody, APG5L antibody, ASP antibody, hAPG5 antibody, ATG5 antibody, CG1643 antibody, DmAtg5 antibody, Dmel\\CG1643 antibody, atg5 antibody, 2010107M05Rik antibody, 3110067M24Rik antibody, AW319544 antibody, Apg5l antibody, Atg5l antibody, C88337 antibody, Paddy antibody, apg5l antibody, zgc:100934 antibody, ATATG5 antibody, AUTOPHAGY 5 antibody, MKP11.20 antibody, MKP11_20 antibody, autophagy related 5 antibody, Autophagy-related 5 antibody, ATG5 autophagy related 5 homolog (S. cerevisiae) antibody, autophagy related 5 L homeolog antibody, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 antibody, autophagy protein Apg5 family antibody, ATG5 antibody, Atg5 antibody, atg5 antibody, atg5.L antibody, APG5 antibody

背景

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

分子量

32447

基因ID

9474

NCBI登录号

NP_004840

UniProt

Q9H1Y0

途径

Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy