UBE2F 抗体 (C-Term)
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- 抗原 See all UBE2F 抗体
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
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抗原表位
- C-Term
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适用
- 人, 小鼠, 大鼠, Cow, 犬
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This UBE2F antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 序列
- C-HLRDKEDFRN KVDD
- 特异性
- This antibody recognizes NCE2 / UBE2F.
- 交叉反应 （详细）
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Species reactivity (expected):Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse. - 纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
- 免疫原
- Peptide with sequence from the C Terminus of the protein sequence according to NP_542409.1. Genename: UBE2F
- Top Product
- Discover our top product UBE2F Primary Antibody
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anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) antibody
UBE2F 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) antibodyUBE2F 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) antibodyUBE2F 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (C-Term) antibodyVerified UBE2F 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (N-Term) antibodyVerified UBE2F 适用: 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (C-Term) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 猴, 兔, 斑马鱼, Bat, Hamster, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (N-Term) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 马, 豚鼠, 猴, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) antibodyUBE2F 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (N-Term) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (Middle Region) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔, 斑马鱼, Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Peptide ELISA: 1/32000. Western Blot: 0.05 - 0.2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- 别名
- NCE2 (UBE2F 产品)
- 别名
- NCE2 antibody, 2510010F15Rik antibody, AI851109 antibody, RGD1307608 antibody, zgc:77005 antibody, ubiquitin conjugating enzyme E2 F (putative) antibody, NEDD8-conjugating enzyme UBE2F antibody, ubiquitin-conjugating enzyme E2F (putative) antibody, ubiquitin conjugating enzyme E2F (putative) S homeolog antibody, UBE2F antibody, Tsp_01616 antibody, ube2f antibody, ube2f.S antibody, Ube2f antibody
- 背景
- NCE2 is a ubiquitin conjugating enzyme. Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.Synonyms: NEDD8-conjugating enzyme 2, UBE2F, Ubiquitin-conjugating enzyme E2 F
- 分子量
- 21.1kDa (Human NP_542409.1 and Mouse NP_080730.1).
- 基因ID
- 140739, 9606
- UniProt
- Q969M7
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