GTPase NRas 抗体 (Internal Region)
Quick Overview for GTPase NRas 抗体 (Internal Region) (ABIN375000)
抗原
See all GTPase NRas (NRAS) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-RMKKLNSSDD GTQ
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特异性
- This antibody detects NRAS.
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Synthetic peptide corresponding to internal sequence of Human NRAS (NP_002515.1). Genename: NRAS
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anti-GTPase NRas (NRAS) (Internal Region) antibody
Verified NRAS 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-GTPase NRas (NRAS) (AA 1-189) antibodyNRAS 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-GTPase NRas (NRAS) (AA 72-101) antibodyNRAS 适用: 人 WB, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-GTPase NRas (NRAS) (AA 147-179) antibodyNRAS 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-GTPase NRas (NRAS) (AA 101-189) antibodyNRAS 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-GTPase NRas (NRAS) (AA 91-140) antibodyNRAS 适用: 人, 大鼠, 小鼠, 兔, Cow, 豚鼠, 马, 斑马鱼, Bat, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GTPase NRas (NRAS) antibodyNRAS 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-GTPase NRas (NRAS) (AA 72-101), (N-Term) antibodyNRAS 适用: 人, 大鼠, 小鼠 WB, FACS, IF 宿主: 兔 Polyclonal RB14459 unconjugated
anti-GTPase NRas (NRAS) (AA 1-186) antibodyNRAS 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GTPase NRas (NRAS) antibodyNRAS 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB56819 unconjugated
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应用备注
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Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.01-0.03 μg/mL. Approx.21 kDa band observed in Human lysates of cell lineA431. In transfected HEK293 transiently expressing NRAS bands of approx.21 kDa and21 kDa are observed. These bands are not observed in the non-transfected HEK293. Immunohistochemistry: 2-3 μg/mL. In paraffin embedded Human Small Intestine shows stainingof cytoplasm and membrane of enterocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- GTPase NRas (NRAS)
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别名
- NRAS
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背景
- NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.Synonyms: ALPS4, GTPase NRas, HRAS1, N-Ras, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog
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分子量
- 21 kDa (NP_002515.1).
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基因ID
- 4893, 9606
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UniProt
- P01111
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途径
- p53 Pathway, MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
抗原
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