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Arylsulfatase B 抗体 (Internal Region)

ARSB 适用: 人 WB, IHC (p) 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN374887
发货至: 中国
  • 抗原 See all Arylsulfatase B (ARSB) 抗体
    Arylsulfatase B (ARSB)
    抗原表位
    • 7
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 35
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    宿主
    • 31
    • 3
    • 1
    山羊
    克隆类型
    • 33
    • 2
    多克隆
    标记
    • 18
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Arylsulfatase B antibody is un-conjugated
    应用范围
    • 25
    • 18
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    序列
    C-KLARGHTNGT KPLD
    特异性
    This antibody detects internal Arylsulfatase B. It is expected to recognise both reported isoforms (NP_000037.2, NP_942002.1).
    交叉反应 (详细)
    Species reactivity (tested):Human
    纯化方法
    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
    免疫原
    Peptide from the internal region of the protein sequence according to NP_000037.2, NP_942002.1.
    Top Product
    Discover our top product ARSB Primary Antibody
  • 应用备注
    Peptide ELISA: limit dilution 1: 64000. Western blot: 0.03-0.1 μg/mL. Approx 45 kDa band observed in Human Heart lysates(calculated MW of 46.5 kDa according to NP_942002.1). An additional band of unknownidentity was also consistently observed at 35 kDa. This band was successfully blocked byincubation with the immunising peptide. We would appreciate any feedback from peoplein the field - have any such results been reported with other antibodies/lysates? Have anyfurther splice variants/modified forms been reported?Immunohistochemistryon paraffin sections: 5-10 μg/mL. In Human Skeletal Muscle showsstriated pattern staining of majority of muscular fibres.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    0.5 mg/mL
    缓冲液
    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  • Zhao, Wang, Li, Dong, Wu: "Arylsulfatase B Mediates the Sulfonation-Transport Interplay in Human Embryonic Kidney 293 Cells Overexpressing Sulfotransferase 1A3." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 44, Issue 9, pp. 1441-9, (2018) (PubMed).

  • 抗原
    Arylsulfatase B (ARSB)
    别名
    Arylsulfatase B (ARSB 产品)
    别名
    ASB antibody, G4S antibody, MPS6 antibody, 1110007C02Rik antibody, AI480648 antibody, As-1 antibody, As-1r antibody, As-1s antibody, As-1t antibody, As1 antibody, As1-r antibody, As1-s antibody, As1-t antibody, Asr-1 antibody, Ast-1 antibody, ARSB antibody, arylsulfatase B antibody, arylsulfatase antibody, arylsulfatase b antibody, ARSB antibody, Arsb antibody, RB348 antibody, LOC5566067 antibody, CpipJ_CPIJ011047 antibody, VDBG_03275 antibody, arsb antibody, LOC5579667 antibody
    背景
    Arylsulfatase B (ARSB) belongs to the sulfatase family. The arylsulfatase B homodimer catalyzes hydrolysis of the sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. Defects in this gene cause Maroteaux-Lamy syndrome.Synonyms: ARSB, ASB, G4S, N-acetylgalactosamine-4-sulfatase
    基因ID
    411, 9606
    UniProt
    P15848
    途径
    Glycosaminoglycan Metabolic Process
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