CD14 抗体 (Internal Region)
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北京 101111
Quick Overview for CD14 抗体 (Internal Region) (ABIN374645)
抗原
See all CD14 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-KRVDADADPR QYAD
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特异性
- This antibody recognizes CD14.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence from the internal region of the protein sequence according to NP_000582.1. Genename: CD14
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anti-CD14 (CD14) (AA 20-214) antibody
CD14 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4B4F12 unconjugated
anti-CD14 (CD14) (AA 20-214) antibodyCD14 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1H5D8 unconjugated
anti-CD14 (CD14) (AA 54-83), (N-Term) antibodyCD14 适用: 人 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14105 unconjugated
anti-CD14 (CD14) (AA 25-148) antibodyCD14 适用: 人 FACS, WB, IHC 宿主: 小鼠 Monoclonal LPSR-2385 unconjugated
anti-CD14 (CD14) antibodyCD14 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1A3B6 unconjugated
anti-CD14 (CD14) (AA 20-345) antibodyCD14 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 14G1F3 unconjugated
anti-CD14 (CD14) (AA 230-345) antibodyCD14 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 1D1F8 unconjugated
anti-CD14 (CD14) (AA 65-327) antibodyCD14 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD14 (CD14) (AA 201-300) antibodyCD14 适用: 人, 小鼠, 大鼠, 犬, 绵羊, 兔 WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD14 (CD14) (Internal Region) antibodyVerified CD14 适用: 人 FACS, WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Peptide ELISA: 1/128000. Western Blot: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: 4 - 6 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD14
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别名
- CD14
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背景
- CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
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基因ID
- 929, 9606
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UniProt
- P08571
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
抗原
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