MDM2 抗体 (Internal Region)
Our Local Distributor
北京 101111
Quick Overview for MDM2 抗体 (Internal Region) (ABIN374627)
抗原
See all MDM2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- Internal Region
-
特异性
- This antibody reacts to MDM2.
-
交叉反应 （详细）
-
Species reactivity (expected):Canine.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
-
免疫原
- Peptide with sequence C-DELSGERQRKRHKSD, from the internal region of the protein sequence
-
-
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 130-230) antibody
MDM2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 101-200) antibodyMDM2 适用: 人 WB, ELISA, IHC (p), RNAi, PLA 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibodyMDM2 适用: 人, 小鼠 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pSer166) antibodyMDM2 适用: 人, 小鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 26-169) antibodyMDM2 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3C1E3 unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 393-424) antibodyMDM2 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pSer186) antibodyMDM2 适用: 人 WB, ELISA, IHC (p), ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 200-430) antibodyMDM2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) antibodyMDM2 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 119-438) antibodyMDM2 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1E6 unconjugated
-
-
应用备注
-
Peptide ELISA: 1/64000. Western Blot: 0.3 - 1.0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 0,5 mg/mL
-
缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
-
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
-
别名
- MDM2
-
背景
- MDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90 kD that forms a complex with the p53 tumor suppressor protein. Human MDM2 was identified as a homologous product of the 'murine double minute 2' gene (mdm2). The MDM2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. Forming a tight complex with the p53 gene, the MDM2 oncogene can inhibit p53 mediated transactivation, MDM2 also binds to p53 protein. Inactivation of tumor suppressor genes leads to deregulated cell proliferation and is a key factor in human tumorigenesis. p53 can be subjected to negative regulation by the product of a single cellular protooncogene. The interference of binding to p53 prevents the interaction of MDM2 and its regulation of the transcriptional activity of p53 in vivo. Direct association of p53 with the cellular protein MDM2 results in ubiquitination and subsequent degradation of p53. MDM2 p53 complexes were preferentially found in S/G2M phases of the cell cycle. The MDM2 gene is alternatively spliced, producing 5 additional splice variant transcripts from the full length MDM2 gene. The alternatively spliced transcripts tend to be expressed in tumorigenic tissue, whereas the full length MDM2 transcript is expressed in normal tissue.Synonyms: Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2
-
基因ID
- 4193, 9606
-
UniProt
- Q00987
-
途径
- p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway
抗原
-
