Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Customer's experiments gave bands at approx 170+90 kDa inlysates of cell line HeLa (calculated MW of 150 kDa according to NP_115979.3). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
KDM2B is a histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylted H3 'Lys-36'. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.Synonyms: CXXC-type zinc finger protein 2, CXXC2, F-box and leucine-rich repeat protein 10, F-box protein FBL10, F-box/LRR-repeat protein 10, FBL10, JEMMA, JHDM1B, JmjC domain-containing histone demethylation protein 1B, Jumonji domain-containing EMSY-interactor methyltransferase motif protein, Lysine-specific demethylase 2B, PCCX2, Protein-containing CXXC domain 2, [Histone-H3]-lysine-36 demethylase 1B