Peptide ELISA: Limit dilution 1: 64000. Western blot: 1-3 μg/mL. Approx 27-30 kDa band observed in human heart and skeletalmuscle lysates (predicted MW of 30 kDa according to NP_006003). Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Liver shows patternedstaining of hepatocyte cytoplasm. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
CLPP belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.Synonyms: Endopeptidase Clp, Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial