KAP1 抗体 (C-Term)
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北京 101111
Quick Overview for KAP1 抗体 (C-Term) (ABIN374296)
抗原
See all KAP1 (TRIM28) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-SSQELSGGPG DGP
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特异性
- This antibody detects TIF1-beta / TRIM28 at C-term.
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交叉反应 （详细）
- Species reactivity (tested):Human and Mouse.
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纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Synthetic peptide from the C-Terminus sequence according to NP_005753.1 Genename: TRIM28
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anti-Tripartite Motif Containing 28 (TRIM28) (AA 576-835) antibody
TRIM28 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibodyVerified TRIM28 适用: 人 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibodyTRIM28 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 4E6 unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) antibodyTRIM28 适用: 人 WB, IF, IP, ICC, IHC (p), ChIP 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (N-Term) antibodyTRIM28 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibodyTRIM28 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1D11 unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (AA 22-291) antibodyTRIM28 适用: 人 WB, IHC, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) antibodyTRIM28 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) antibodyTRIM28 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (Center) antibodyTRIM28 适用: 人 IHC 宿主: 兔 Monoclonal HMV335 unconjugated Recombinant Antibody
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应用备注
- Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.2-1 μg/mL. Approx 100-120 kDa band seen in HepG2 lysate. Immunohistochemistry: 5 μg/mL (Ssuccessfully used on Human gastric cancer surgical
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "Comments on: Improving breast cancer surgery: a classification and quadrant per quadrant atlas for oncoplastic surgery (Clough KB, Kaufman GJ, et al. ASO DOI 10.1245/s10434-009-0792-y)." in: Annals of surgical oncology, Vol. 18 Suppl 3, pp. S257-8; author reply S259-60, (2011) (PubMed).
: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." in: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, (2010) (PubMed).
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: "Comments on: Improving breast cancer surgery: a classification and quadrant per quadrant atlas for oncoplastic surgery (Clough KB, Kaufman GJ, et al. ASO DOI 10.1245/s10434-009-0792-y)." in: Annals of surgical oncology, Vol. 18 Suppl 3, pp. S257-8; author reply S259-60, (2011) (PubMed).
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- KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
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别名
- TIF1-beta / TRIM28
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背景
- The KRAB (Kruppel Associated Box) domain is minimally about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB zinc finger protein (KRAB ZFP) genes have been identified in the human genome. These proteins functionally repress transcription via specific interactions with KAP1 (KRAB Associated Protein 1). KAP1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP1 has been shown to form complexes with KRAB domain transcription factors and increase the efficiency with which they mediate repression. KAP1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel associated box containing zinc finger protein 1). KAP1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, helping to regulate transcriptional repression. Recent studies have shown that KAP1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.Synonyms: KAP-1, KAP1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, KRIP1, Nuclear corepressor KAP-1, RING finger protein 96, RNF96, TIF1 beta, TIF1B, Transcription intermediary factor 1-beta, Tripartite motif-containing protein 28
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基因ID
- 10155
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NCBI登录号
- NP_005753
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UniProt
- Q13263
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途径
- Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus
抗原
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