Peptide ELISA: Limit dilution 1: 8000. Western blot: 0.5-1.5 μg/mL. Approx.55-60 kDa band observed in lysates of Jurkat andHepG2 (calculated MW of 51.0 kDa according to NP_005375.2). The observed molecularweight corresponds to earlier findings in literature (Ikushima et al. , Proc Natl Acad Sci U SA.1997 Mar 18,94(6): 2609-14. , PMID: 9122243). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
NFIL3 is a basic leucine zipper transcription factor which activates IL3 gene expression and can also repress transcription.Synonyms: E4BP4, IL3BP1, NF-IL3A, NFIL3, Nuclear factor interlE4 promoter binding-protein 4