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RAF1 抗体 (pSer642)
RAF1
适用: 人, 小鼠, 大鼠, Cow, 小鸡, 犬, Non-Human Primate
WB
宿主: 兔
Polyclonal
unconjugated
RAF1抗体详情
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抗原
See all RAF1 抗体
RAF1
(V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
抗原表位
All epitopes for RAF1 抗体
pSer642
适用
All reactivities for RAF1 抗体
人, 小鼠, 大鼠, Cow, 小鸡, 犬, Non-Human Primate
宿主
All hosts for RAF1 抗体
兔
克隆类型
All clonalities for RAF1 抗体
多克隆
标记
All conjugates for RAF1 抗体
This RAF1 antibody is un-conjugated
应用范围
All applications for RAF1 抗体
Western Blotting (WB)
特异性
This antibody recognizes the ~74k Raf-1 protein phosphorylated at Ser642.
交叉反应 (详细)
Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse and non-Human Primates. Species reactivity (tested):Rat.
纯化方法
Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
免疫原
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser642 of rat Raf1.
亚型
IgG
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Alternatives
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使用细节
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应用备注
Western blot: 1/1,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
贮存及处理
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状态
Liquid
缓冲液
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody undiluted (in aliquots) at-20 °C.
RAF1目标详情
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抗原
RAF1
(V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
别名
RAF1 (RAF1 产品 )
别名
CRAF antibody, NS5 antibody, Raf-1 antibody, c-Raf antibody, c-raf antibody, craf antibody, raf1 antibody, RAF1 antibody, 6430402F14Rik antibody, AA990557 antibody, BB129353 antibody, Craf1 antibody, D830050J10Rik antibody, v-Raf antibody, raf antibody, cRaf antibody, Raf-1 proto-oncogene, serine/threonine kinase antibody, Raf-1 proto-oncogene, serine/threonine kinase a antibody, v-raf-leukemia viral oncogene 1 antibody, Raf-1 proto-oncogene, serine/threonine kinase S homeolog antibody, RAF1 antibody, Raf1 antibody, raf1a antibody, raf1 antibody, raf1.S antibody
背景
The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002, Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
基因ID
24703
NCBI登录号
NP_036771
UniProt
P11345
途径
MAPK Pathway , RTK signaling , Fc-epsilon Receptor Signaling Pathway , Neurotrophin Signaling Pathway , cAMP Metabolic Process , Stem Cell Maintenance , Hepatitis C , Autophagy , Signaling of Hepatocyte Growth Factor Receptor , VEGF Signaling , BCR Signaling
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