FBXW7 抗体 (Internal Region)
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- 抗原 See all FBXW7 抗体
- FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This FBXW7 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- 品牌
- IHC-plus™
- 特异性
- Recognizes human Cdc4 protein at ~60-80kD. Multiple bands may represent various isoforms.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide derived from an internal region of the human and mouse Cdc4 (archipelago (AGO), F-box/WD-repeat protein 7, F-box and WD-40 domain protein 7, F-box protein FBW7, FBX30 and SEL-10).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product FBXW7 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), IP, WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation: 7 μg/reaction. Positive control: Human T47D, U2-OS, MCF-7, HepG2 and HeLa cell lysates. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))
- 别名
- FBXW7 / FBW7 (FBXW7 产品)
- 背景
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Name/Gene ID: FBXW7
Family: F-Box
Synonyms: FBXW7, AGO, Archipelago homolog, Archipelago, CDC4, F-box protein FBW7, FBXW6, F-box protein FBX30, F-box protein SEL-10, HCdc4, Homolog of C elegans sel-10, FBW6, FBX30, FBW7, HAgo, SEL-10, SEL10 - 基因ID
- 55294
- UniProt
- Q969H0
- 途径
- Notch Signaling, EGFR Signaling Pathway
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