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- 抗原 See all MDM2 抗体
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
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抗原表位
- pSer166
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MDM2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- 特异性
- The antibody detects endogenous level of MDM2 only when phosphorylated at serine 166.
- 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- 免疫原
- Peptide sequence around phosphorylation site of pSer166 (A-I-S (p) -E-T) derived from Human MDM2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- 亚型
- IgG
- Top Product
- Discover our top product MDM2 Primary Antibody
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- 应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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Pathological signaling via platelet-derived growth factor receptor {alpha} involves chronic activation of Akt and suppression of p53." in: Molecular and cellular biology, Vol. 31, Issue 9, pp. 1788-99, (2011) (PubMed).
: "N-acetylcysteine suppresses retinal detachment in an experimental model of proliferative vitreoretinopathy." in: The American journal of pathology, Vol. 177, Issue 1, pp. 132-40, (2010) (PubMed).
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Pathological signaling via platelet-derived growth factor receptor {alpha} involves chronic activation of Akt and suppression of p53." in: Molecular and cellular biology, Vol. 31, Issue 9, pp. 1788-99, (2011) (PubMed).
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- 抗原
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
- 别名
- MDM2 (MDM2 产品)
- 别名
- ACTFS antibody, HDMX antibody, hdm2 antibody, zgc:109834 antibody, 1700007J15Rik antibody, AA415488 antibody, Mdm-2 antibody, MDM2 proto-oncogene antibody, MDM2 proto-oncogene S homeolog antibody, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) antibody, MDM2 oncogene, E3 ubiquitin protein ligase antibody, transformed mouse 3T3 cell double minute 2 antibody, MDM2 antibody, mdm2.S antibody, Mdm2 antibody, mdm2 antibody
- 背景
- This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues
- 分子量
- 90 kDa
- NCBI登录号
- NP_002383
- UniProt
- Q00987
- 途径
- p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway
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