(1 reference)-
- 抗原 See all H2AFX 抗体
- H2AFX (H2A Histone Family, Member X (H2AFX))
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抗原表位
- pSer139
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This H2AFX antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- The antibody detects endogenous level of Histone H2A.X only when phosphorylated at serine 139.
- 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- 免疫原
- Peptide sequence around phosphorylation site of pSer139 (Q-A-S (p) -Q-E) derived from Human Histone H2A.X. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- 亚型
- IgG
- Top Product
- Discover our top product H2AFX Primary Antibody
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anti-H2A Histone Family, Member X (H2AFX) antibody
H2AFX 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) antibodyH2AFX 适用: 人 WB, IHC, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) (AA 115-143), (C-Term) antibodyH2AFX 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 938CT5-1-1 unconjugated
anti-H2A Histone Family, Member X (H2AFX) (pSer140) antibodyH2AFX 适用: 人 WB, ELISA, IHC (p), ICC, FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) (ubLys119) antibodyH2AFX 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) antibodyH2AFX 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 8C5 unconjugated
anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibodyH2AFX 适用: 人 WB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibodyH2AFX 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibodyH2AFX 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member X (H2AFX) (C-Term) antibodyH2AFX 适用: 人 WB, IF, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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: "HSF4 is involved in DNA damage repair through regulation of Rad51." in: Biochimica et biophysica acta, Vol. 1822, Issue 8, pp. 1308-15, (2012) (PubMed).
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: "HSF4 is involved in DNA damage repair through regulation of Rad51." in: Biochimica et biophysica acta, Vol. 1822, Issue 8, pp. 1308-15, (2012) (PubMed).
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- 抗原
- H2AFX (H2A Histone Family, Member X (H2AFX))
- 别名
- Histone H2A.X (H2AFX 产品)
- 别名
- H2A.X antibody, H2A/X antibody, H2AX antibody, AW228881 antibody, H2ax antibody, Hist5-2ax antibody, gammaH2ax antibody, zgc:56329 antibody, h2a.x antibody, h2a/x antibody, h2ax antibody, RGD1566119 antibody, h2a antibody, h2afx antibody, H2A histone family member X antibody, H2A histone family, member X antibody, H2A histone family member X L homeolog antibody, histone cluster 1, H2ah antibody, histone cluster 2, H2ab S homeolog antibody, histone H2AX antibody, H2AFX antibody, H2afx antibody, h2afx antibody, h2afx.L antibody, HIST1H2AH antibody, hist2h2ab.S antibody, LOC100522201 antibody, LOC100720536 antibody
- 背景
- Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
- 分子量
- 15 kDa
- NCBI登录号
- NP_002096
- UniProt
- P16104
- 途径
- Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
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