The antibody detects endogenous level of Raf1 only when phosphorylated at serine 338.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pSer338 (R-D-S (p) -S-Y) derived from Human RAF. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.