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MAP2K4 抗体 (pSer80)

This anti-MAP2K4 antibody is a 兔 多克隆 antibody detecting MAP2K4 in WB, IHC 和 IF. Suitable for 人, 小鼠 和 大鼠. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN362192
发货至: 中国

Quick Overview for MAP2K4 抗体 (pSer80) (ABIN362192)

抗原

See all MAP2K4 抗体
MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

适用

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人, 小鼠, 大鼠

宿主

  • 149
  • 12

克隆类型

  • 150
  • 12
多克隆

标记

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  • 1
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This MAP2K4 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
  • 抗原表位

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    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    pSer80

    特异性

    The antibody detects endogenous level of SEK1/MKK4 only when phosphorylated at serine 80.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    免疫原

    Peptide sequence around phosphorylation site of pSer80 (T-H-S (p) -I-E) derived from Human SEK1, MKK4. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

    亚型

    IgG
  • 应用备注

    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    Immunofluorescence: 1:100-1:200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Saha, Jiang, Yang, Zhu, Wang, Schimmer, Qiu, Chang: "Targeting p53 via JNK pathway: a novel role of RITA for apoptotic signaling in multiple myeloma." in: PLoS ONE, Vol. 7, Issue 1, pp. e30215, (2012) (PubMed).

  • 抗原

    MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

    别名

    SEK1/MKK4

    背景

    Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).

    分子量

    44 kDa

    NCBI登录号

    NP_003001

    UniProt

    P45985

    途径

    MAPK Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling
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