AKT2 抗体 (pSer474)
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Quick Overview for AKT2 抗体 (pSer474) (ABIN362103)
抗原
See all AKT2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer474
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特异性
- The antibody detects endogenous level of Akt2 only when phosphorylated at serine 474.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pSer474 (Q-F-S (p) -Y-S) derived from Human Akt2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
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别名
- Akt2
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背景
- General protein kinase capable of phosphorylating several known proteins.
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分子量
- 60 kDa
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UniProt
- P31751
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途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, TLR signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
抗原
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