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ATF2 抗体 (pThr53, pThr71)

This 兔 多克隆 antibody specifically detects ATF2 in WB 和 IHC. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN361933
发货至: 中国
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Quick Overview for ATF2 抗体 (pThr53, pThr71) (ABIN361933)

抗原

See all ATF2 抗体
ATF2 (Activating Transcription Factor 2 (ATF2))

适用

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人, 小鼠, 大鼠

宿主

  • 366
  • 15
  • 1
  • 1

克隆类型

  • 325
  • 58
多克隆

标记

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This ATF2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 抗原表位

    • 54
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    • 15
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    • 2
    • 1
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    pThr53, pThr71

    特异性

    The antibody detects endogenous level of ATF-2 only when phosphorylated at threonine 71 or 53.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    免疫原

    Peptide sequence around phosphorylation site of pThr71 or 53 (T-P-T (p) -P-T) derived from Human ATF2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

    亚型

    IgG
  • 应用备注

    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • 抗原

    ATF2 (Activating Transcription Factor 2 (ATF2))

    别名

    ATF2

    背景

    Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

    分子量

    65-75 kDa

    NCBI登录号

    NP_001871

    UniProt

    P15336

    途径

    MAPK Pathway, RTK signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades
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