DNA Damage 抗体
适用: 所有种类
ICC, IF, IHC, DB, IP, ELISA, FACS, Func
宿主: 小鼠
Monoclonal
15A3
unconjugated
(7 references)-
- 抗原
- DNA Damage
- 适用
- 所有种类
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Dot Blot (DB), Immunoprecipitation (IP), ELISA, Flow Cytometry (FACS), Functional Studies (Func)
- 特异性
- Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
- 纯化方法
- Protein G Purified
- 免疫原
- 8-hydroxy-guanosine-BSA and -casein conjugates
- 克隆位点
- 15A3
- 亚型
- IgG2b
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- 应用备注
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- IHC (1:1000)
- optimal dilutions for assays should be determined by the user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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: "Long-Term Adverse Effects of Oxidative Stress on Rat Epididymis and Spermatozoa." in: Antioxidants (Basel, Switzerland), Vol. 9, Issue 2, (2020) (PubMed).
: "In vivo oxidative stress alters thiol redox status of peroxiredoxin 1 and 6 and impairs rat sperm quality." in: Asian journal of andrology, Vol. 19, Issue 1, pp. 73-79, (2017) (PubMed).
: "Loss of angiotensin II receptor expression in dopamine neurons in Parkinson's disease correlates with pathological progression and is accompanied by increases in Nox4- and 8-OH guanosine-related ..." in: Acta neuropathologica communications, Vol. 3, pp. 9, (2015) (PubMed).
: "Advancing age increases sperm chromatin damage and impairs fertility in peroxiredoxin 6 null mice." in: Redox biology, Vol. 5, pp. 15-23, (2015) (PubMed).
: "Neuroprotective effect of sulforaphane against methylglyoxal cytotoxicity." in: Chemical research in toxicology, Vol. 28, Issue 6, pp. 1234-45, (2015) (PubMed).
: "Epilepsy: neuroinflammation, neurodegeneration, and APOE genotype." in: Acta neuropathologica communications, Vol. 1, pp. 41, (2013) (PubMed).
: "Disruption of redox homeostasis leads to oxidative DNA damage in spermatocytes of Wolbachia-infected Drosophila simulans." in: Insect molecular biology, Vol. 21, Issue 5, pp. 510-20, (2012) (PubMed).
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: "Long-Term Adverse Effects of Oxidative Stress on Rat Epididymis and Spermatozoa." in: Antioxidants (Basel, Switzerland), Vol. 9, Issue 2, (2020) (PubMed).
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- 抗原
- DNA Damage
- 物质类
- Chemical
- 背景
- DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage(1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).
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