(1 reference)-
- 抗原
- PAK1-3
- 抗原表位
- pThr402
- 适用
- 大鼠
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Western Blotting (WB)
- 特异性
- Specific for the ~68k to ~70k PAK protein phosphorylated at Thr402. The immunolabeling of PAK is completely eliminated by (-phosphatase treatment.
- 交叉反应
- 大鼠
- 预测反应
- bovine, canine, human, mouse
- 纯化方法
- Antigen Affinity Purified from Pooled Serum
- 免疫原
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 conjugated to KLH
-
- 应用备注
- Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
- 储存条件
- -20 °C
-
-
: "Synapses of amphids defective (SAD-A) kinase promotes glucose-stimulated insulin secretion through activation of p21-activated kinase (PAK1) in pancreatic β-Cells." in: The Journal of biological chemistry, Vol. 287, Issue 31, pp. 26435-44, (2012) (PubMed).
-
: "Synapses of amphids defective (SAD-A) kinase promotes glucose-stimulated insulin secretion through activation of p21-activated kinase (PAK1) in pancreatic β-Cells." in: The Journal of biological chemistry, Vol. 287, Issue 31, pp. 26435-44, (2012) (PubMed).
-
- 抗原
- PAK1-3
- 背景
- In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: a-PAK (also known as PAK-1) and -PAK (also known as PAK-3) are mostly brain-specific, while -PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003, Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000). Anti-Phospho-Thr402 PAK-1,2,3 Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68k to ~70k PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr402 PAK-1,2,3 antibody. The immunolabeling of PAK is completely eliminated by treatment with (-Ptase.
- 分子量
- 68/70 kDa
- 基因ID
- 29431
- UniProt
- P35465
-
You are here:
