Immunohistochemistry for Formalin-Fixed Paraffin Embedded (FFPE) Tissue Sections andFrozen Sections: Dilute 1/100-1/200 using polymer or streptavidin approx. Biotin system for 30 mins at RT. This antibody requires antigen retriever (boiling FFPE tissue sections in 10 mMcitrate buffer, pH 6.0 for 10-20 mins. followed by cooling at RT for 20 mins). Positive Control: Human pancreas or GI tumor. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
3.0 mg/mL
缓冲液
10 mM PO4, 150 mM NaCl, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Synaptophysin is a synaptic vesicle glycoprotein with 4 transmembrane domain MW 38 kDa. Thisproteinis present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is also marker of endocrine tumors. It is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms.Synonyms: Major synaptic vesicle protein p38, Neuroendocrine Marker