Collagen Type I 抗体
Quick Overview for Collagen Type I 抗体 (ABIN344731)
抗原
See all Collagen Type I (COL1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes both native and heat denatured human collagen type I. No cross-reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5 % binding to collagens II and III in ELISA, RIA or immunoblot.
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纯化方法
- Ammonium sulfate precipitation
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免疫原
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Human Collagen Type I.
Type of Immunogen: Purified protein -
亚型
- IgG1
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应用备注
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Approved: ELISA (10 - 20 μg/mL), IHC, IHC-Fr, RIA
Usage: Suitable for use in ELISA, Immunohistochemistry and RIA. Not suitable for Western Blot. ELISA: Use as capture or as a second detecting antibody in sandwich format (Apparent binding constant is ~0.1nM). Immunohistochemistry (acetone or ethanol fixed frozen 10um sections): 10-20 μg/mL, Staining is similar to that for polyclonal antibodies to interstitial collagen I. Not suitable for paraffin-embedded sections or formaldehyde fixation.
Not recommended for: IHC-P, WB -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute, 1 mL sterile distilled water or sterile 40-50 % glycerol.
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浓度
- Lot specific
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缓冲液
- Lyophilized powder. Reconstitute, 1 mL sterile distilled water or sterile 40-50 % glycerol.
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- Collagen Type I (COL1) (Collagen, Type I (COL1))
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别名
- Collagen I
抗原
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