CD14 抗体
Quick Overview for CD14 抗体 (ABIN343180)
抗原
See all CD14 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Membrane bound and soluble mouse CD14. The antibody blocks LPS binding to membrane bound CD14.
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纯化方法
- Protein A affinity column
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免疫原
- Monocytes of mouse
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亚型
- IgG1
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应用备注
- Suitable for immunostaining of CD14 positive cells and inhibition studies Binds at LPS activated mouse monocytes as well as mouse CD14 transfected cells or recombinant mouse CD14 ELISA. Inhibition of LPS binding to CD14 (Binding at LPS binding site). Up to 1 ug/ml biG53 inhibit binding of FITC-LPS (0.5ug/ml) to 6 x 105 CD14+CHO transfectants (FACS), Binding titre at mouse CD14 transfected CHO-cells: more than 20.000 (ELISA, Flow cytometry)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with bidistilled water
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH 7.16
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储存液
- Without preservative
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储存条件
- -20 °C,-80 °C
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- CD14
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别名
- CD14
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背景
- Background: biG 53 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.
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基因ID
- 12475
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NCBI登录号
- NP_033971
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UniProt
- P10810
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
抗原
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