CD14 抗体
Quick Overview for CD14 抗体 (ABIN343179)
抗原
See all CD14 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recombinant mouse CD14. The antibody don't blocks LPS binding to membrane bound CD14.
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纯化方法
- Protein G affinity column
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免疫原
- recombinant mouse CD14
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亚型
- IgG1
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应用备注
- ELISA , immunostaining of mouse macrophages after stimulation 6h with LPS, Western blot with recombinant mouse CD14. Binding titre at mouse CD14 transfected CHO-cells: more than 100.000 (ELISA, Flow cytometry), no binding at LPS-binding site, endotoxin content less than 0.1ng/ml
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with bidistilled water
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH 7.15
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储存液
- Without preservative
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储存条件
- -20 °C,-80 °C
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- CD14
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别名
- CD14
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背景
- Background: biG 57 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.
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分子量
- ~50kDa
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基因ID
- 12475
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NCBI登录号
- NP_033971
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UniProt
- P10810
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
抗原
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