PIK3CA 抗体 (AA 878-891)
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Quick Overview for PIK3CA 抗体 (AA 878-891) (ABIN337177)
抗原
See all PIK3CA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 878-891
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特异性
- Human PIK3CA.
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum (100%) Turkey, Chicken, Platypus, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish (93%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide C-HQWLKDKNKGEIYD from an internal region of human PIK3CA (NP_006209.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum (100%), Turkey, Chicken, Platypus, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish (93%).
Type of Immunogen: Synthetic peptide
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应用备注
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Approved: IHC, IHC-P (2.5 - 5 μg/mL), Peptide-ELISA (1:128000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
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- PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))
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别名
- PIK3CA / PI3K
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背景
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Name/Gene ID: PIK3CA
Family: Non-Protein Kinase
Synonyms: PIK3CA, MCAP, p110-alpha, p110alpha, PI3K, PI3-kinase p110 subunit alpha, PI3-kinase subunit alpha, PI3Kalpha, MCM, MCMTC, PI3K-alpha, PtdIns-3-kinase subunit alpha, CLOVE -
基因ID
- 5290
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NCBI登录号
- NP_006209
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UniProt
- P42336
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途径
- PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling
抗原
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