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PIK3CA 抗体 (AA 961-1068)

Name: PIK3CA 抗体 (AA 961-1068)
SKU: ABIN677198
Availability: OutOfStock

PIK3CA 适用: 人, 小鼠, 大鼠, Cow, 兔 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

HeLa Cell lysates probed with PI3KCA Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PI3KCA Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PI3KCA Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

3 images

(1 reference)

产品编号 ABIN677198

发货至: 中国

Datasheet as PDF

抗原 See all PIK3CA 抗体

PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))
抗原表位
15

14

13

11

9

7

5

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 961-1068
适用
79

57

31

21

14

1
人, 小鼠, 大鼠, Cow, 兔
宿主
82

5

2

1
兔
Compatible Secondaries
克隆类型
78

12
多克隆
标记
29

8

7

6

4

3

3

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1
This PIK3CA antibody is un-conjugated
应用范围
69

32

25

25

25

11

5

4

2

2

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

交叉反应

Cow, 人, 小鼠, 兔, 大鼠

预测反应

Cow,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human PI3KCA

亚型

IgG

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PIK3CA 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody
PIK3CA 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 1019-1050), (C-Term) antibody
PIK3CA 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB01702 unconjugated

anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody
PIK3CA 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 504-533) antibody
PIK3CA 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (pTyr317) antibody
PIK3CA 适用: 人, 小鼠, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (Internal Region) antibody
Verified PIK3CA 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (N-Term) antibody
PIK3CA 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 1-206), (Catalytic Subunit alpha) antibody
PIK3CA 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (Catalytic Subunit alpha) antibody
PIK3CA 适用: 人 WB, ELISA, IF 宿主: 兔 Monoclonal 10E5 unconjugated Recombinant Antibody

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Paul-Samojedny, Suchanek, Borkowska, Pude?ko, Owczarek, Kowalczyk, Machnik, Fila-Dani?ow, Kowalski: "Knockdown of AKT3 (PKB?) and PI3KCA suppresses cell viability and proliferation and induces the apoptosis of glioblastoma multiforme T98G cells." in: BioMed research international, Vol. 2014, pp. 768181, (2014) (PubMed).
抗原

PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

别名

PI3KCA (PIK3CA 产品)

别名

CLOVE antibody, CWS5 antibody, MCAP antibody, MCM antibody, MCMTC antibody, PI3K antibody, p110-alpha antibody, C-P3K antibody, PI3-kinase antibody, PI3K-alpha antibody, pi3-k antibody, pi3k antibody, AA414921 antibody, C530050K14 antibody, p50alpha antibody, p55alpha antibody, p85alpha antibody, 6330412C24Rik antibody, caPI3K antibody, p110 antibody, p110alpha antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha antibody, Phosphoinositide-3-kinase, catalytic, alpha polypeptide antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha antibody, phosphoinositide-3-kinase regulatory subunit 1 antibody, PIK3CA antibody, GL50803_14855 antibody, pik3ca antibody, Pik3ca antibody, Pik3r1 antibody

背景

Synonyms: MCM, CWS5, MCAP, PI3K, CLOVE, MCMTC, p11-alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase 11 kDa catalytic subunit alpha, PtdIns-3-kinase subunit p11-alpha, p11alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, PIK3CA
Background: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS.

基因ID

5290

UniProt

P42336

途径

PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling